Transposon mutagenesis identifies genes essential for Plasmodium falciparum gametocytogenesis

Ikadai H.; Saliba K.S.; Kanzok S.M.McLean K.J.Tanaka T.Q.Cao J.Williamson K.C.Jacobs-Lorena M.

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Transposon mutagenesis identifies genes essential for Plasmodium falciparum gametocytogenesis

机译：Transposon mutagenesis identifies genes essential for Plasmodium falciparum gametocytogenesis

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Gametocytes are essential for Plasmodium transmission, but little is known about the mechanisms that lead to their formation. Using piggyBac transposon-mediated insertional mutagenesis, we screened for parasites that no longer form mature gametocytes, which led to the isolation of 29 clones (insertional gametocyte-deficient mutants) that fail to form mature gametocytes. Additional analysis revealed 16 genes putatively responsible for the loss of gametocytogenesis, none of which has been previously implicated in gametocytogenesis. Transcriptional profiling and detection of an early stage gametocyte antigen determined that a subset of these mutants arrests development at stage I or in early stage II gametocytes, likely representing genes involved in gametocyte maturation. The remaining mutants seem to arrest before formation of stage I gametocytes and may represent genes involved in commitment to the gametocyte lineage.

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《Proceedings of the National Academy of Sciences of the United States of America》 |2013年第18期|e1676-e1684|共2页
作者
Ikadai H.; Saliba K.S.; Kanzok S.M.McLean K.J.Tanaka T.Q.Cao J.Williamson K.C.Jacobs-Lorena M.;
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作者单位

Department of Molecular Microbiology and Immunology, Johns Hopkins Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, United States;

Department of Biology, Loyola University, Chicago, IL 60660, United States;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
Gametocyte development; Malaria; PiggyBac insertional mutagenesis; Plasmodium genetics;

Transposon mutagenesis identifies genes essential for Plasmodium falciparum gametocytogenesis

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