Incomplete Wood–Ljungdahl pathway facilitates one-carbon metabolism in organohalide-respiring Dehalococcoides mccartyi

Wei-Qin Zhuang; Shan Yi; Markus BillVanessa L. BrissonXueyang FengYujie MenMark E. ConradYinjie J. TangLisa Alvarez-Cohen

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Incomplete Wood–Ljungdahl pathway facilitates one-carbon metabolism in organohalide-respiring Dehalococcoides mccartyi

机译：Incomplete Wood–Ljungdahl pathway facilitates one-carbon metabolism in organohalide-respiring Dehalococcoides mccartyi

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The acetyl-CoA “Wood–Ljungdahl” pathway couples the folatemediated one-carbon (C1) metabolism to either CO_2 reduction or acetate oxidation via acetyl-CoA. This pathway is distributed in diverse anaerobes and is used for both energy conservation and assimilation of C1 compounds. Genome annotations for all sequenced strains of Dehalococcoides mccartyi, an important bacterium involved in the bioremediation of chlorinated solvents, reveal homologous genes encoding an incomplete Wood–Ljungdahl pathway. Because this pathway lacks key enzymes for both C1 metabolism and CO_2 reduction, its cellular functions remain elusive. Here we used D. mccartyi strain 195 as a model organism to investigate the metabolic function of this pathway and its impacts on the growth of strain 195. Surprisingly, this pathway cleaves acetyl-CoA to donate a methyl group for production of methyltetrahydrofolate(CH_3-THF) for methionine biosynthesis, representing an unconventional strategy for generating CH_3-THF in organisms without methylene-tetrahydrofolate reductase. Carbon monoxide(CO) was found to accumulate as an obligate by-product from the acetyl-CoA cleavage because of the lack of a CO dehydrogenase in strain 195. CO accumulation inhibits the sustainable growth and dechlorination of strain 195 maintained in pure cultures, but can be prevented by CO-metabolizing anaerobes that coexist with D. mccartyi, resulting in an unusual syntrophic association. We also found that this pathway incorporates exogenous formate to support serine biosynthesis. This study of the incomplete Wood–Ljungdahl pathway in D. mccartyi indicates a unique bacterial C1 metabolism that is critical for D. mccartyi growth and interactions in dechlorinating communities andmay play a role in other anaerobic communities.

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《Proceedings of the National Academy of Sciences of the United States of America》 |2014年第17期|6419-6424|共6页
作者
Wei-Qin Zhuang; Shan Yi; Markus BillVanessa L. BrissonXueyang FengYujie MenMark E. ConradYinjie J. TangLisa Alvarez-Cohen;
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作者单位

Energy, Environmental and Chemical Engineering, Washington University, St. Louis, MO 63130;

Department of Civil and Environmental Engineering, University of California, Berkeley, CA 94720-1710;

Earth Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
reductive dechlorination; ~(13)C isotope analysis; acetyl-CoA synthase;

Incomplete Wood–Ljungdahl pathway facilitates one-carbon metabolism in organohalide-respiring Dehalococcoides mccartyi

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