Smoke-derived karrikin perception by the α/β-hydrolase KAI2 from arabidopsis

Guo Y.; Zheng Z.; La Clair J.J.Chory J.Noel J.P.

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Smoke-derived karrikin perception by the α/β-hydrolase KAI2 from arabidopsis

机译：Smoke-derived karrikin perception by the α/β-hydrolase KAI2 from arabidopsis

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Genetic studies in Arabidopsis implicate an α/β-hydrolase, KARRIKININSENSITIVE 2 (KAI2) as a receptor for karrikins, germination-promoting butenolide small molecules found in the smoke of burned plants. However, direct biochemical evidence for the interaction between KAI2 and karrikin and for the mechanism of downstream signaling by a KAI2-karrikin complex remain elusive. We report crystallographic analyses and ligand-binding experiments for KAI2 recognition of karrikins. The karrikin-1 (KAR1) ligand sits in the opening to the active site abutting a helical domain insert but distal from the canonical catalytic triad (Ser95-His246-Asp217) of α/β-hydrolases, consistent with the lack of detectable hydrolytic activity by purified KAI2. The closest approach of KAR1 to Ser95-His246-Asp217 is 3.8 ? from His246. Six aromatic side chains, including His246, encapsulate KAR1 through geometrically defined aromatic-aromatic interactions. KAR1 binding induces a conformational change in KAI2 at the active site entrance. A crevice of hydrophobic residues linking the polar edge of KAR1 and the helical domain insert suggests that KAI2-KAR1 creates a contiguous interface for binding signaling partners in a liganddependent manner.

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《Proceedings of the National Academy of Sciences of the United States of America》 |2013年第20期|8284-8289|共6页
作者
Guo Y.; Zheng Z.; La Clair J.J.Chory J.Noel J.P.;
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作者单位

Howard Hughes Medical Institute, Salk Institute for Biological Studies, San Diego, CA 92037, United States;

Department of Chemistry and Biochemistry, University of California, San Diego, CA 92093, United States;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
Plant signaling; Seed dormancy; Wildfires;

Smoke-derived karrikin perception by the α/β-hydrolase KAI2 from arabidopsis

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