Structural and functional analysis of an anchorless fibronectin-binding protein FBPS from Gram-positive bacterium Streptococcus suis

Musyoki Abednego Moki; Shi Zhongyu; Xuan ChunlingLu GuangwenQi JianxunGao FengZheng BeiwenZhang QiangminLi YanHaywood JoelLiu CuihuaYan JinghuaShi YiGao George F.

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Structural and functional analysis of an anchorless fibronectin-binding protein FBPS from Gram-positive bacterium Streptococcus suis

机译：Structural and functional analysis of an anchorless fibronectin-binding protein FBPS from Gram-positive bacterium Streptococcus suis

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The anchorless fibronectin-binding proteins (FnBPs) are a group of important virulence factors for which the structures are not available and the functions are not well defined. In this study we performed comprehensive studies on a prototypic member of this group: the fibronectin-/fibrinogen-binding protein from Streptococcus suis (FBPS). The structures of the N-and C-terminal halves (FBPS-N and FBPS-C), which together cover the full-length protein in sequence, were solved at a resolution of 2.1 and 2.6 angstrom, respectively, and each was found to be composed of two domains with unique folds. Furthermore, we have elucidated the organization of these domains by small-angle X-ray scattering. We further showed that the fibronectin-binding site is located in FBPS-C and that FBPS promotes the adherence of S. suis to host cells by attaching the bacteria via FBPS-N. Finally, we demonstrated that FBPS functions both as an adhesin, promoting S. suis attachment to host cells, and as a bacterial factor, activating signaling pathways via beta 1 integrin receptors to induce chemokine production.

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《Proceedings of the National Academy of Sciences of the United States of America.》 |2016年第48期|13869-13874|共6页
作者
Musyoki Abednego Moki; Shi Zhongyu; Xuan ChunlingLu GuangwenQi JianxunGao FengZheng BeiwenZhang QiangminLi YanHaywood JoelLiu CuihuaYan JinghuaShi YiGao George F.;
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作者单位

Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol, Beijing 100101, Peoples R China;

Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China;

Zhejiang Univ, Sch Med, Affiliated Hosp 1, State Key Lab Diag & Treatment Infect Dis, Hangzhou 310003, Zhejiang, Peoples R China;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
fibronectin-binding protein of Streptococcus suis; structure; novel fold; fibronectin-binding property; function;

Structural and functional analysis of an anchorless fibronectin-binding protein FBPS from Gram-positive bacterium Streptococcus suis

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