Measurement of urinary calcium using AT89C51RD2 microcontroller

P. Neelamegam; A. Jamaludeen; A. RajendranR. Raghunathan

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Measurement of urinary calcium using AT89C51RD2 microcontroller

机译：Measurement of urinary calcium using AT89C51RD2 microcontroller

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A simple and inexpensive absorption technique for determination of calcium ion in urine samples is developed, comprising a light emitting diode (650 nm) as the light source and photodiode as the detector with AT89C51RD2 microcontroller. The design of the system and details of interface, calibration, and procedure of operation are explained in this paper. Software is developed to monitor sample processing and to display the results in liquid crystal display screen. With 15 (mu)l sample volume, a linear output is obtained in the range of 2.5-7.5 mM calcium with a detection limit of 0.06 mM. Interferences from other cations such as monovalent ion and divalent ion are investigated in the expected range, which are normally present in clinical samples, and absorption changes over the pH range of 3-12 are also determined. This system has been demonstrated successfully for the successive assay of calcium in urine samples, with the results comparing well to those achieved and in good agreement with values obtained with the current clinical spectrophotometric method at 95percent of confidence level.

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《Review of Scientific Instruments》 |2009年第4期|044704-1-044704-5|共5页
作者
P. Neelamegam; A. Jamaludeen; A. RajendranR. Raghunathan;
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Department of Electronics and Instrumentation Engineering, SASTRA University, Thanjavur 613 402, Tamil Nadu, India;

Department of Electronics, St. Joseph's College (Autonomous), Tiruchirappalli 620 002, Tamil Nadu, India;

PG and Research Department of Physics, Nehru Memorial College (Autonomous), Puthanampatti, Tiruchirappalli 621 007, Tamil Nadu, IndiaPG and Research Department of Physics, AVVM Sri Pushpam College (Autonomous), Poondi, Thanjavur, Tamil Nadu 613 503, India;

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Measurement of urinary calcium using AT89C51RD2 microcontroller

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