PCL Nanopillars Versus Nanofibers: A Contrast in Progenitor Cell Morphology, Proliferation, and Fate Determination

Ashok Prasad; Dustin Berger; Ketul Popat

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PCL Nanopillars Versus Nanofibers: A Contrast in Progenitor Cell Morphology, Proliferation, and Fate Determination

机译：PCL Nanopillars Versus Nanofibers: A Contrast in Progenitor Cell Morphology, Proliferation, and Fate Determination

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The role of nanotopography on the long-term response of progenitor cells is explored using polycaprolactone (PCL) nanopillar and nanofiber surfaces seeded with plastic-adherent rat multipotent mesenchymal stromal cells (MSCs). After 4 weeks in culture under normal expansion media conditions, MSCs cultured on nanofibers exhibit better adherence, increased proliferation, and maintain increasingly dense fibroblast-like morphologies. In contrast, MSCs seeded on nanopillar surfaces display lowered adherence, reduced proliferation, and adopt highly elongated cellular morphologies. Immunofluorescent staining of MSCs on PCL nanopillars reveals the presence of two bone marker proteins, osteopontin and osteocalcin, providing evidence for surface induced differentiation into osteoblast-like cells. Unlike the nanopillar topography, MSCs cultured on nanofiber and smooth PCL surfaces did not appear to undergo osteogenesis. Observed differences in cellular response to the PCL nanotopographies offer strategies to direct progenitor cell populations solely based upon submicron surface modifications. This study provides a foundation for future work exploring variations in PCL nanopillar topography with the goal of optimizing adherence and osteogenic response of MSCs.

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《Advanced Engineering Materials》 |2012年第6期|B351-B356|共6页
作者
Ashok Prasad; Dustin Berger; Ketul Popat;
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Department of Chemical and Biological Engineering, School of Biomedical Engineering, Colorado State University;

School of Biomedical Engineering, Colorado State University;

Department of Mechanical Engineering, School of Biomedical Engineering, Colorado State University;

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正文语种英语
中图分类工程材料学;
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PCL Nanopillars Versus Nanofibers: A Contrast in Progenitor Cell Morphology, Proliferation, and Fate Determination

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