• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Food science and biotechnology >Megaprimer PCR for site-directed mutagenesis of cyclodextrin glucanotransferase
【24h】

Megaprimer PCR for site-directed mutagenesis of cyclodextrin glucanotransferase

机译：Megaprimer PCR for site-directed mutagenesis of cyclodextrin glucanotransferase

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相关主题

摘要

Site-directed mutagenesis of the Bacillus macerans cyclodextrin glucanotransferase (cgt) gene was performed using a modified method of the megaprimer polymerase chain reaction. Relatively longer PCR products were synthesized between 816 and 1,211 bpsusing mis-matched internal primer during die first round reaction, and these megaprimers were used to successfully amplify the cgt gene. To avoid a sequence-shift caused by non-specific addition of adenine at 3' end of PCR product, a primer was designed. Even with the constraint, this method extends the PCR technique in producing internal or multiple site mutations.

著录项

获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号