A comparative immunocytochemical study using an antiserum against a synthetic analogue of the corpora cardiaca peptide Pea-CAH-I (MI, neurohormone D) ofPeriplaneta americana

M.Eckert; J.Gabriel; H.BirkenbeilG.GreinerJ.RapusG.Gäde

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A comparative immunocytochemical study using an antiserum against a synthetic analogue of the corpora cardiaca peptide Pea-CAH-I (MI, neurohormone D) ofPeriplaneta americana

机译：A comparative immunocytochemical study using an antiserum against a synthetic analogue of the corpora cardiaca peptide Pea-CAH-I (MI, neurohormone D) ofPeriplaneta americana

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An antiserum against the octapeptide Pea-CAH-I, a member of the adipokinetic hormone/red pigment-concentrating hormone family, has been produced for immunocytochemical staining in insects and various other invertebrate species. The anti-Pea-CAH-I serum stains the glandular corpora cardiaca cells of those insect species that synthesize identical or structurally similar peptides. In the corpora cardiaca of species producing peptides with a different C-terminus, these cells remain unstained. Pea-CAH-I-like immunoreactivity has also been found in neurons of the central nervous system of all invertebrate orders studied. The antiserum recognizes the C-terminal sequence Pro-Asn-Trp-NH2of the Pea-CAH-I molecule as established by enzyme immunoassay. The widespread Pea-CAH-I-like immunoreactivity in all nervous systems of the studied animals probably does not reflect the presence of Pea-CAH-I but the occurrence of peptides carrying similar epitopes.

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《cell and tissue research》 |1996年第3期|401-413|共页
作者
M.Eckert; J.Gabriel; H.BirkenbeilG.GreinerJ.RapusG.Gäde;
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Institut für Allgemeine Zoologie und Tierphysiologie, F.- Schiller-Universität, Erbertstrasse 1, D-07743 Jena, Germany;

Sächsische Akademie der Wissenschaften zu Leipzig, D-07743 Jena, Germany;

Institut für Allgemeine Biochemie, F.-Schiller-Universität, D-07743 Jena, GermanyZoology Department, University of Cape Town, Cape Town, South Africa;

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A comparative immunocytochemical study using an antiserum against a synthetic analogue of the corpora cardiaca peptide Pea-CAH-I (MI, neurohormone D) ofPeriplaneta americana

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