Isolation and characterization of new microsatellite markers from the Japanese scallop (Patinopecten yessoensis)

Chen M.; Chang Y.-Q.; Ding J.Sun X.-W.

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Isolation and characterization of new microsatellite markers from the Japanese scallop (Patinopecten yessoensis)

机译：Isolation and characterization of new microsatellite markers from the Japanese scallop (Patinopecten yessoensis)

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摘要

Twenty polymorphic microsatellite loci were isolated from the Japanese scallop (Patinopecten yessoensis) using a method of hybridizing twice. The genetic diversity was analyzed by these microsatellite markers in field specimens from Zhangzidao Island in Liaoning Province, China. Observed heterozygosity (H_o), expected heterozygosity (H_e), number of effective alleles (N_e), and polymorphism information contents (PIC) were calculated. The heterozygote deficiency or excess was detected by the fixation index (Fis). The Hardy-Weinberg equilibrium was tested by possibilities (P_(HWE)) using chi-square test. Results indicate that the average of allele numbers was 6. 0. The mean values of the parameters H_o, H_e, N_e, and PIC were 0. 7921, 0. 7076, 4. 4347, and 0. 6684, respectively. Only 4 of 20 microsatellite markers showed heterozygote deficiency including loci FJ262370, FJ262381, FJ262384, and FJ262400. The P_(HWE) value indicates the wild population deviated somewhat from the Hardy-Weinberg equilibrium. These newly isolated markers increase the available molecular resources of the Japanese scallop.

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《Conservation genetics》 |2010年第3期|1139-1142|共4页
作者
Chen M.; Chang Y.-Q.; Ding J.Sun X.-W.;
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作者单位

Key Laboratory of Mariculture, DaLian Fisheries University, 116023 DaLian, Liaoning, China;

Heilongjiang Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China;

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正文语种英语
中图分类遗传学;
关键词
Japanese scallop; Microsatellite; Population structure; Twice hybridizations;

Isolation and characterization of new microsatellite markers from the Japanese scallop (Patinopecten yessoensis)

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