Disease resistance through impairment of alpha-SNAP-NSF interaction and vesicular trafficking by soybean Rhg1

Bayless Adam M.; Smith John M.; Song JunqiMcMinn Patrick H.Teillet AliceAugust Benjamin K.Bent Andrew F.

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Disease resistance through impairment of alpha-SNAP-NSF interaction and vesicular trafficking by soybean Rhg1

机译：Disease resistance through impairment of alpha-SNAP-NSF interaction and vesicular trafficking by soybean Rhg1

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alpha-SNAP soluble NSF (N-ethylmaleimide-sensitive factor) attachment protein and NSF proteins are conserved across eukaryotes and sustain cellular vesicle trafficking by mediating disassembly and reuse of SNARE protein complexes, which facilitate fusion of vesicles to target membranes. However, certain haplotypes of the Rhg1 (resistance to Heterodera glycines 1) locus of soybean possess multiple repeat copies of an alpha-SNAP gene (Glyma. 18G022500) that encodes atypical amino acids at a highly conserved functional site. These Rhg1 loci mediate resistance to soybean cyst nematode (SCN; H. glycines), the most economically damaging pathogen of soybeans worldwide. Rhg1 is widely used in agriculture, but the mechanisms of Rhg1 disease resistance have remained unclear. In the present study, we found that the resistance-type Rhg1 alpha-SNAP is defective in interaction with NSF. Elevated in planta expression of resistance-type Rhg1 alpha-SNAPs depleted the abundance of SNARE-recycling 20S complexes, disrupted vesicle trafficking, induced elevated abundance of NSF, and caused cytotoxicity. Soybean, due to ancient genome duplication events, carries other loci that encode canonical (wild-type) alpha-SNAPs. Expression of these a-SNAPs counteracted the cytotoxicity of resistance-type Rhg1 alpha-SNAPs. For successful growth and reproduction, SCN dramatically reprograms a set of plant root cells and must sustain this sedentary feeding site for 2-4 weeks. Immunoblots and electron microscopy immunolocalization revealed that resistance-type alpha-SNAPs specifically hyperaccumulate relative to wild-type a-SNAPs at the nematode feeding site, promoting the demise of this biotrophic interface. The paradigm of disease resistance through a dysfunctional variant of an essential gene may be applicable to other plant-pathogen interactions.

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《Proceedings of the National Academy of Sciences of the United States of America.》 |2016年第47期|E7375-E7382|共8页
作者
Bayless Adam M.; Smith John M.; Song JunqiMcMinn Patrick H.Teillet AliceAugust Benjamin K.Bent Andrew F.;
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作者单位

Univ Wisconsin, Dept Plant Pathol, Madison, WI 53706 USA;

Univ Wisconsin, Sch Med & Publ Hlth, Electron Microscopy Facil, Madison, WI 53706 USA;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
plant disease resistance; alpha-SNAP; soybean cyst nematode; Rhg1;

Disease resistance through impairment of alpha-SNAP-NSF interaction and vesicular trafficking by soybean Rhg1

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