Structural basis for the enhancement of virulence by viral spindles and their in vivo crystallization

Chiu Elaine; Hijnen Marcel; Bunker Richard D.Boudes MarionRajendran ChitraAizel KaheinaOlieric VincentSchulze-Briese ClemensMitsuhashi WataruYoung VivienneWard Vernon K.Bergoin MaxMetcalf PeterCoulibaly Fasseli

首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America. >Structural basis for the enhancement of virulence by viral spindles and their in vivo crystallization

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Structural basis for the enhancement of virulence by viral spindles and their in vivo crystallization

机译：Structural basis for the enhancement of virulence by viral spindles and their in vivo crystallization

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The great benefits that chemical pesticides have brought to agriculture are partly offset by widespread environmental damage to nontarget species and threats to human health. Microbial bioinsecticides are considered safe and highly specific alternatives but generally lack potency. Spindles produced by insect poxviruses are crystals of the fusolin protein that considerably boost not only the virulence of these viruses but also, in cofeeding experiments, the insecticidal activity of unrelated pathogens. However, the mechanisms by which spindles assemble into ultra-stable crystals and enhance virulence are unknown. Here we describe the structure of viral spindles determined by X-ray microcrystallography from in vivo crystals purified from infected insects. We found that a C-terminal molecular arm of fusolin mediates the assembly of a globular domain, which has the hallmarks of lytic polysaccharide monooxy-genases of chitinovorous bacteria. Explaining their unique stability, a 3D network of disulfide bonds between fusolin dimers covalently crosslinks the entire crystalline matrix of spindles. However, upon ingestion by a new host, removal of the molecular arm abolishes this stabilizing network leading to the dissolution of spindles. The released monooxygenase domain is then free to disrupt the chitin-rich peritrophic matrix that protects insects against oral infections. The mode of action revealed here may guide the design of potent spindles as synergetic additives to bioinsecticides.

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《Proceedings of the National Academy of Sciences of the United States of America.》 |2015年第13期|3973-3978|共6页
作者
Chiu Elaine; Hijnen Marcel; Bunker Richard D.Boudes MarionRajendran ChitraAizel KaheinaOlieric VincentSchulze-Briese ClemensMitsuhashi WataruYoung VivienneWard Vernon K.Bergoin MaxMetcalf PeterCoulibaly Fasseli;
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作者单位

Monash Univ, Sch Biomed Sci, Dept Biochem & Mol Biol, Clayton, Vic 3800, Australia;

Paul Scherrer Inst, Swiss Light Source, CH-5232 Villigen, Switzerland;

Natl Inst Agrobiol Sci, Tsukuba, Ibaraki 3058634, JapanUniv Otago, Sch Med Sci, Dept Microbiol & Immunol, Dunedin 9054, New ZealandUniv Montpellier 2, F-34095 Montpellier, FranceUniv Auckland, Sch Biol Sci, Auckland 1010, New Zealand;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
microcrystallography; in vivo crystallization; poxvirus; LPMO; pesticide;

Structural basis for the enhancement of virulence by viral spindles and their in vivo crystallization

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