Architecture of the yeast Rrp44-exosome complex suggests routes of RNA recruitment for 3 ' end processing

Wang  HW; Wang  J; Ding  FCallahan  KBratkowski  MAButler  JSNogales  EKe  A

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Architecture of the yeast Rrp44-exosome complex suggests routes of RNA recruitment for 3 ' end processing

机译：Architecture of the yeast Rrp44-exosome complex suggests routes of RNA recruitment for 3 ' end processing

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The eukaryotic core exosome (CE) is a conserved nine-subunit protein complex important for 3' end trimming and degradation of RNA. In yeast, the Rrp44 protein constitutively associates with the CE and provides the sole source of processive 3'-to-5' exoribonuclease activity. Here we present EM reconstructions of the core and Rrp44-bound exosome complexes. The two-lobed Rrp44 protein binds to the RNase PH domain side of the exosome and buttresses the bottom of the exosome-processing chamber. The Rrp44 C-terminal body part containing an RNase II-type active site is anchored to the exosome through a conserved set of interactions mainly to the Rrp45 and Rrp43 subunit, whereas the Rrp44 N-terminal head part is anchored to the Rrp41 subunit and may function as a roadblock to restrict access of RNA to the active site in the body region. The Rrp44-exosorne (RE) architecture suggests an active site sequestration mechanism for strict control of 3' exoribonuclease activity in the RE complex.

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《Proceedings of the National Academy of Sciences of the United States of America》 |2007年第43期|16844-16849|共6页
作者
Wang HW; Wang J; Ding FCallahan KBratkowski MAButler JSNogales EKe A;
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作者单位

Univ Calif Berkeley, Lawrence Berkeley Lab, Div Life Sci, Berkeley, CA 94720 USA;

Cornell Univ, Dept Mol Biol & Genet, Ithaca, NY 14853 USA;

Univ Rochester, Sch Med, Dept Microbiol, Rochester, NY 14642 USAUniv Rochester, Sch Med, Dept Immunol, Rochester, NY 14642 USAUniv Rochester, Sch Med, Dept Biochem, Rochester, NY 14642 USAUniv Rochester, Sch Med, Dept Biophys, Rochester, NY 14642 USAUniv Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USAUniv Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
electron microscopy; single-particle reconstruction; exoribonuclease; MESSENGER-RNA; STRUCTURAL BASIS; ENDONUCLEOLYTIC CLEAVAGE; ARCHAEAL EXOSOME; NUCLEAR EXOSOME; QUALITY-CONTROL; POLYMERASE-II; SKI COMPLEX; DEGRADATION; SUBUNIT;

Architecture of the yeast Rrp44-exosome complex suggests routes of RNA recruitment for 3 ' end processing

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