Stoichiometry-focused (18)F-labeling of alkyne-substituted oligodeoxynucleotides using azido(((18)F)fluoromethyl)benzenes by Cu-catalyzed Huisgen reaction.

Kuboyama T; Nakahara M; Yoshino MCui YSako TWada YImanishi TObika SWatanabe YSuzuki MDoi H

首页> 外文期刊>Bioorganic and medicinal chemistry >Stoichiometry-focused (18)F-labeling of alkyne-substituted oligodeoxynucleotides using azido(((18)F)fluoromethyl)benzenes by Cu-catalyzed Huisgen reaction.

【24h】

Stoichiometry-focused (18)F-labeling of alkyne-substituted oligodeoxynucleotides using azido(((18)F)fluoromethyl)benzenes by Cu-catalyzed Huisgen reaction.

机译：Stoichiometry-focused (18)F-labeling of alkyne-substituted oligodeoxynucleotides using azido(((18)F)fluoromethyl)benzenes by Cu-catalyzed Huisgen reaction.

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

A novel method for (18)F-radiolabeling of oligodeoxynucleotides (ODNs) by a Cu-catalyzed Huisgen reaction has been developed by using the lowest possible amount of the precursor biomolecule for the realization of stoichiometry-oriented PET (positron emission tomography) chemistry. Under the optimized cyclization conditions of p- or m-azido((18)Ffluoromethyl)benzene and alkyne-substituted ODN (20nmol) at 40 degrees C for 15min in the presence of CuSO(4), TBTA tris((1-benzyl-1H-1,2,3-triazol-4-yl)methyl)amine, and sodium ascorbate (2:1:2), the synthesis of (18)F-labeled ODNs with sufficiently high radioactivities of 2.1-2.5GBq and specific radioactivities of 1800-2400GBq/mumol have been accomplished for use in animal and human PET studies.

著录项

来源
《Bioorganic and medicinal chemistry》 |2011年第1期|249-255|共7页
作者
Kuboyama T; Nakahara M; Yoshino MCui YSako TWada YImanishi TObika SWatanabe YSuzuki MDoi H;
展开▼
作者单位

Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.;

展开▼
收录信息
原文格式 PDF
正文语种英语
中图分类药学;
关键词

Stoichiometry-focused (18)F-labeling of alkyne-substituted oligodeoxynucleotides using azido(((18)F)fluoromethyl)benzenes by Cu-catalyzed Huisgen reaction.

摘要

著录项

相关主题

期刊订阅