Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis

Sivan G.; Martin S.E.; Myers T.G.Buehler E.Szymczyk K.H.Ormanoglu P.Moss B.

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Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis

机译：Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis

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Poxviruses are considered less dependent on host functions than other DNA viruses because of their cytoplasmic site of replication and large genomes, which encode enzymes for DNA and mRNA synthesis. Nevertheless, RNAi screens with two independent human genome-scale libraries have identified more than 500 candidate genes that significantly inhibited and a similar number that enhanced replication and spread of infectious vaccinia virus (VACV). Translational, ubiquitin-proteosome, and endoplasmic reticulum-to-Golgi transport functions, known to be important for VACV, were enriched in the siRNA-inhibiting group, and RNA polymerase II and associated functions were enriched in the siRNAenhancing group. Additional findings, notably the inhibition of VACV spread by siRNAs to several nuclear pore genes, were unanticipated. Knockdown of nucleoporin 62 strongly inhibited viral morphogenesis, with only a modest effect on viral gene expression, recapitulating and providing insight into previous studies with enucleated cells.

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《Proceedings of the National Academy of Sciences of the United States of America》 |2013年第9期|3519-3524|共6页
作者
Sivan G.; Martin S.E.; Myers T.G.Buehler E.Szymczyk K.H.Ormanoglu P.Moss B.;
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作者单位

Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, United States;

Division of Preclinical Innovation, National Center for Advancing Translational Sciences, National Institutes of Health, Bethesda, MD 20892, United States;

Genomic Technologies Section, Research Technologies Branch, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892, United States;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
Nucleoporin 62; Poxvirus replication; Poxvirus-host interactions; Syntaxin 5; Vaccinia virus replication;

Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis

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