Interkingdom metabolic transformations captured by microbial imaging mass spectrometry

Moree W.J.; Phelan V.V.; Wu C.-H.Bandeira N.Cornett D.S.Duggan B.M.Dorrestein P.C.

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Interkingdom metabolic transformations captured by microbial imaging mass spectrometry

机译：Interkingdom metabolic transformations captured by microbial imaging mass spectrometry

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In polymicrobial infections, microbes can interact with both the host immune system and one another through direct contact or the secretion of metabolites, affecting disease progression and treatment options. The thick mucus in the lungs of patients with cystic fibrosis is highly susceptible to polymicrobial infections by opportunistic pathogens, including the bacterium Pseudomonas aeruginosa and the fungus Aspergillus fumigatus. Unravelling the hidden molecular interactions within such polymicrobial communities and their metabolic exchange processes will require effective enabling technologies applied to model systems. In the present study, MALDI-TOF and MALDI-FT-ICR imaging mass spectrometry (MALDI-IMS) combined with MS/MS networking were used to provide insight into the interkingdom interaction between P. aeruginosa and A. fumigatus at the molecular level. The combination of these technologies enabled the visualization and identification of metabolites secreted by these microorganisms grown on agar. A complex molecular interplay was revealed involving suppression, increased production, and biotransformation of a range of metabolites. Of particular interest is the observation that P. aeruginosa phenazine metabolites were converted by A. fumigatus into other chemical entities with alternative properties, including enhanced toxicities and the ability to induce fungal siderophores. This work highlights the capabilities of MALDI-IMS and MS/MS network analysis to study interkingdom interactions and provides insight into the complex nature of polymicrobial metabolic exchange and biotransformations.

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《Proceedings of the National Academy of Sciences of the United States of America》 |2012年第34期|13811-13816|共6页
作者
Moree W.J.; Phelan V.V.; Wu C.-H.Bandeira N.Cornett D.S.Duggan B.M.Dorrestein P.C.;
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Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California at San Diego, San Diego, CA 92093, United States;

Bruker Daltonics, Billerica, MA 01821, United States;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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正文语种英语
中图分类自然科学总论;
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Interkingdom metabolic transformations captured by microbial imaging mass spectrometry

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