Entirely plasmid-based reverse genetics system for rotaviruses

Kanai Yuta; Komoto Satoshi; Kawagishi TakahiroNouda RyotaroNagasawa NaokoOnishi MisaMatsuura YoshiharuTaniguchi KokiKobayashi Takeshi

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Entirely plasmid-based reverse genetics system for rotaviruses

机译：Entirely plasmid-based reverse genetics system for rotaviruses

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Rotaviruses (RVs) are highly important pathogens that cause severe diarrhea among infants and young children worldwide. The understanding of the molecular mechanisms underlying RV replication and pathogenesis has been hampered by the lack of an entirely plasmid-based reverse genetics system. In this study, we describe the recovery of recombinant RVs entirely from cloned cDNAs. The strategy requires coexpression of a small transmembrane protein that accelerates cell-to-cell fusion and vaccinia virus capping enzyme. We used this system to obtain insights into the process by which RV nonstructural protein NSP1 subverts host innate immune responses. By insertion into the NSP1 gene segment, we recovered recombinant viruses that encode split-green fluorescent protein-tagged NSP1 and NanoLuc luciferase. This technology will provide opportunities for studying RV biology and foster development of RV vaccines and therapeutics.

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《Proceedings of the National Academy of Sciences of the United States of America.》 |2017年第9期|2349-2354|共6页
作者
Kanai Yuta; Komoto Satoshi; Kawagishi TakahiroNouda RyotaroNagasawa NaokoOnishi MisaMatsuura YoshiharuTaniguchi KokiKobayashi Takeshi;
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作者单位

Osaka Univ, Microbial Dis Res Inst, Dept Mol Virol, Suita, Osaka 5650871, Japan;

Fujita Hlth Univ, Dept Virol & Parasitol, Sch Med, Toyoake, Aichi 4701192, Japan;

Osaka Univ, Microbial Dis Res Inst, Int Res Ctr Infect Dis, Lab Viral Replicat, Suita, Osaka 5650871, Japan;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
rotavirus; reverse genetics; vaccine; reporter virus;

Entirely plasmid-based reverse genetics system for rotaviruses

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