Quantifying single-cell secretion in real time using resonant hyperspectral imaging

Juan-Colas Jose; Hitchcock Ian S.; Coles MarkJohnson StevenKrauss Thomas F.

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Quantifying single-cell secretion in real time using resonant hyperspectral imaging

机译：Quantifying single-cell secretion in real time using resonant hyperspectral imaging

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Cell communication is primarily regulated by secreted proteins, whose inhomogeneous secretion often indicates physiological disorder. Parallel monitoring of innate protein-secretion kinetics from individual cells is thus crucial to unravel systemic malfunctions. Here, we report a label-free, high-throughput method for parallel, in vitro, and real-time analysis of specific single-cell signaling using hyperspectral photonic crystal resonant technology. Heterogeneity in physiological thrombopoietin expression from individual HepG2 liver cells in response to platelet desialylation was quantified demonstrating how mapping real-time protein secretion can provide a simple, yet powerful approach for studying complex physiological systems regulating protein production at single-cell resolution.

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《Proceedings of the National Academy of Sciences of the United States of America.》 |2018年第52期|13204-13209|共6页
作者
Juan-Colas Jose; Hitchcock Ian S.; Coles MarkJohnson StevenKrauss Thomas F.;
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作者单位

Univ York, Dept Phys, York YO10 5DD, N Yorkshire, England;

Univ York, Dept Biol, York YO10 5DD, N Yorkshire, England;

Univ Oxford, Kennedy Inst Rheumatol, Oxford OX3 7FY, EnglandUniv York, Dept Elect Engn, York YO10 5DD, N Yorkshire, England;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
single-cell analysis; label-free; photonic biosensing; photonic crystal;

Quantifying single-cell secretion in real time using resonant hyperspectral imaging

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