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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America. >Glycine receptor alpha 3 and alpha 2 subunits mediate tonic and exogenous agonist-induced currents in forebrain
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Glycine receptor alpha 3 and alpha 2 subunits mediate tonic and exogenous agonist-induced currents in forebrain

机译：Glycine receptor alpha 3 and alpha 2 subunits mediate tonic and exogenous agonist-induced currents in forebrain

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Neuronal inhibition can occur via synaptic mechanisms or through tonic activation of extrasynaptic receptors. In spinal cord, glycine mediates synaptic inhibition through the activation of heteromeric glycine receptors (GlyRs) composed primarily of alpha 1 and beta subunits. Inhibitory GlyRs are also found throughout the brain, where GlyR alpha 2 and alpha 3 subunit expression exceeds that of alpha 1, particularly in forebrain structures, and coassembly of these alpha subunits with the beta subunit appears to occur to a lesser extent than in spinal cord. Here, we analyzed GlyR currents in several regions of the adolescent mouse forebrain (striatum, prefrontal cortex, hippocampus, amygdala, and bed nucleus of the stria terminalis). Our results show ubiquitous expression of GlyRs that mediate large-amplitude currents in response to exogenously applied glycine in these forebrain structures. Additionally, tonic inward currents were also detected, but only in the striatum, hippocampus, and prefrontal cortex (PFC). These tonic currents were sensitive to both strychnine and picrotoxin, indicating that they are mediated by extrasynaptic homomeric GlyRs. Recordings from mice deficient in the GlyR alpha 3 subunit (Glra3(-/-)) revealed a lack of tonic GlyR currents in the striatum and the PFC. In Glra2(-/Y) animals, GlyR tonic currents were preserved; however, the amplitudes of current responses to exogenous glycine were significantly reduced. We conclude that functional alpha 2 and alpha 3 GlyRs are present in various regions of the forebrain and that alpha 3 GlyRs specifically participate in tonic inhibition in the striatum and PFC. Our findings suggest roles for glycine in regulating neuronal excitability in the forebrain.

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