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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Global characterization of in vivo enzyme catalytic rates and their correspondence to in vitro k(cat) measurements
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Global characterization of in vivo enzyme catalytic rates and their correspondence to in vitro k(cat) measurements

机译：Global characterization of in vivo enzyme catalytic rates and their correspondence to in vitro k(cat) measurements

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摘要

Turnover numbers, also known as k(cat) values, are fundamental properties of enzymes. However, k(cat) data are scarce and measured in vitro, thus may not faithfully represent the in vivo situation. A basic question that awaits elucidation is: how representative are k(cat) values for the maximal catalytic rates of enzymes in vivo? Here, we harness omics data to calculate k(max)(vivo), the observed maximal catalytic rate of an enzyme inside cells. Comparison with k(cat) values from Escherichia coli, yields a correlation of r(2) = 0.62 in log scale (p < 10(-10)), with a root mean square difference of 0.54 (3.5-fold in linear scale), indicating that in vivo and in vitro maximal rates generally concur. By accounting for the degree of saturation of enzymes and the backward flux dictated by thermodynamics, we further refine the correspondence between k(max)(vivo) and k(cat) values. The approach we present here characterizes the quantitative relationship between enzymatic catalysis in vitro and in vivo and offers a high-throughput method for extracting enzyme kinetic constants from omics data.

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