Sulfenome mining in Arabidopsis thaliana

Cezary Waszczak; Salma Akter; Dominique EeckhoutGeert PersiauKhadija WahniNandita BodraInge Van MolleBarbara De SmetDidier VertommenKris GevaertGeert De JaegerMarc Van MontaguJoris MessensFrank Van Breusegem

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Sulfenome mining in Arabidopsis thaliana

机译：Sulfenome mining in Arabidopsis thaliana

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Reactive oxygen species (ROS) have been shown to be potent signaling molecules. Today, oxidation of cysteine residues is a well-recognized posttranslational protein modification, but the signaling processes steered by such oxidations are poorly understood. To gain insight into the cysteine thiol-dependent ROS signaling in Arabidopsis thaliana, we identified the hydrogen peroxide (H2O2)-dependent sulfenome: that is, proteins with at least one cysteine thiol oxidized to a sulfenic acid. By means of a genetic construct consisting of a fusion between the C-terminal domain of the yeast (Saccharomyces cerevisiae) AP-1-like (YAP1) transcription factor and a tandem affinity purification tag, we detected ~100 sulfenylated proteins in Arabidopsis cell suspensions exposed to H_2O_2 stress. The in vivo YAP1-based trapping of sulfenylated proteins was validated by a targeted in vitro analysis of DEHYDROASCORBATE REDUCTASE2 (DHAR2). In DHAR2, the active site nucleophilic cysteine is regulated through a sulfenic acid-dependent switch, leading to S-glutathionylation, a protein modification that protects the protein against oxidative damage.

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《Proceedings of the National Academy of Sciences of the United States of America》 |2014年第31期|11545-11550|共6页
作者
Cezary Waszczak; Salma Akter; Dominique EeckhoutGeert PersiauKhadija WahniNandita BodraInge Van MolleBarbara De SmetDidier VertommenKris GevaertGeert De JaegerMarc Van MontaguJoris MessensFrank Van Breusegem;
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作者单位

Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium;

Structural Biology Research Center, VIB, 1050 Brussels, Belgium;

de Duve Institute, Université Catholique de Louvain, 1200 Brussels, BelgiumDepartment of Medical Protein Research, VIB, 9000 Ghent, Belgium;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
oxidative stress; redox regulation; cysteine oxidation;

Sulfenome mining in Arabidopsis thaliana

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