iPSCs and fibroblast subclones from the same fibroblast population contain comparable levels of sequence variations

Kwon Erika M.; Connelly John P.; Hansen Nancy F.Donovan Frank X.Winkler ThomasDavis Brian W.Alkadi HalahChandrasekharappa Settara C.Dunbar Cynthia E.Mullikin James C.Liu Paul

首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America. >iPSCs and fibroblast subclones from the same fibroblast population contain comparable levels of sequence variations

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iPSCs and fibroblast subclones from the same fibroblast population contain comparable levels of sequence variations

机译：iPSCs and fibroblast subclones from the same fibroblast population contain comparable levels of sequence variations

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Genome integrity of induced pluripotent stem cells (iPSCs) has been extensively studied in recent years, but it is still unclear whether iPSCs contain more genomic variations than cultured somatic cells. One important question is the origin of genomic variations detected in iPSCs-whether iPSC reprogramming induces such variations. Here, we undertook a unique approach by deriving fibroblast subclones and clonal iPSC lines from the same fibroblast population and applied next-generation sequencing to compare genomic variations in these lines. Targeted deep sequencing of parental fibroblasts revealed that most variants detected in clonal iPSCs and fibroblast subclones were rare variants inherited from the parental fibroblasts. Only a small number of variants remained undetectable in the parental fibroblasts, which were thus likely to be de novo. Importantly, the clonal iPSCs and fibroblast subclones contained comparable numbers of de novo variants. Collectively, our data suggest that iPSC reprogramming is not mutagenic.

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《Proceedings of the National Academy of Sciences of the United States of America.》 |2017年第8期|1964-1969|共6页
作者
Kwon Erika M.; Connelly John P.; Hansen Nancy F.Donovan Frank X.Winkler ThomasDavis Brian W.Alkadi HalahChandrasekharappa Settara C.Dunbar Cynthia E.Mullikin James C.Liu Paul;
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作者单位

NHGRI, Oncogenesis & Dev Sect, NIH, Bethesda, MD 20892 USA;

NHGRI, Comparat Genom Unit, NIH Intramural Sequencing Ctr, NIH, Bethesda, MD 20892 USA;

NHGRI, Genom Core, NIH, Bethesda, MD 20892 USANHLBI, Hematol Branch, NIH, Bethesda, MD 20892 USANHGRI, Canc Genet & Comparat Genom Branch, Bethesda, MD 20892 USA;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
iPSCs; fibroblasts; reprogramming; genomic variation; exome sequencing;

iPSCs and fibroblast subclones from the same fibroblast population contain comparable levels of sequence variations

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