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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Developmental propagation of V(D)J recombinationassociated DNA breaks and translocations in mature B cells via dicentric chromosomes
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Developmental propagation of V(D)J recombinationassociated DNA breaks and translocations in mature B cells via dicentric chromosomes

机译：Developmental propagation of V(D)J recombinationassociated DNA breaks and translocations in mature B cells via dicentric chromosomes

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Mature IgM~+ B-cell lymphomas that arise in certain ataxia telangiectasia- mutated (ATM)-deficient compound mutant mice harbor translocations that fuse V(D)J recombination-initiated IgH doublestrand breaks (DSBs) on chromosome 12 to sequences downstream of c-myc on chromosome 15, generating dicentric chromosomes and c-myc amplification via a breakage-fusion-bridge mechanism. As V(D)J recombination DSBs occur in developing progenitor B cells in the bone marrow, we sought to elucidate a mechanism by which such DSBs contribute to oncogenic translocations/amplifications in mature B cells. For this purpose, we applied high-throughput genome-wide translocation sequencing to study the fate of introduced c-myc DSBs in splenic IgM~+ B cells stimulated for activationinduced cytidine deaminase (AID)-dependent IgH class switch recombination (CSR). We found frequent translocations of c-myc DSBs to AID-initiated DSBs in IgH switch regions in wild-type and ATM-deficient B cells. However, c-myc also translocated frequently to newly generated DSBs within a 35-Mb region downstream of IgH in ATM-deficient, but not wild-type, CSR-activated B cells. Moreover, we found suchDSBs and translocations in activated B cells that did not express AID or undergo CSR. Our findings indicate that ATM deficiency leads to formation of chromosome 12 dicentrics via recombinationactivating gene-initiated IgH DSBs in progenitor B cells and that these dicentrics can be propagated developmentally into mature B cells where they generate new DSBs downstream of IgH via breakagefusion- bridge cycles. We propose that dicentrics formed by joining V(D)J recombination–associated IgH DSBs to DSBs downstream of c-myc in ATM-deficient B lineage cells similarly contribute to c-myc amplification and mature B-cell lymphomas.

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