Single-Cell Imaging of HIV-1 Provirus (SCIP)

Di Primio C.; Quercioli V.; Allouch A.Gijsbers R.Christ F.Debyser Z.Arosio D.Cereseto A.

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Single-Cell Imaging of HIV-1 Provirus (SCIP)

机译：Single-Cell Imaging of HIV-1 Provirus (SCIP)

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Recent advances in fluorescence microscopy provided tools for the investigation and the analysis of the viral replication steps in the cellular context. In the HIV field, the current visualization systems successfully achieve the fluorescent labeling of the viral envelope and proteins, but not the genome. Here, we developed a system able to visualize the proviral DNA of HIV-1 through immunofluorescence detection of repair foci for DNA double-strand breaks specifically induced in the viral genome by the heterologous expression of the I-SceI endonuclease. The system for Single-Cell Imaging of HIV-1 Provirus, named SCIP, provides the possibility to individually track integrated-viral DNA within the nuclei of infected cells. In particular, SCIP allowed us to perform a topological analysis of integrated viral DNA revealing that HIV-1 preferentially integrates in the chromatin localized at the periphery of the nuclei.

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《Proceedings of the National Academy of Sciences of the United States of America》 |2013年第14期|5636-5641|共6页
作者
Di Primio C.; Quercioli V.; Allouch A.Gijsbers R.Christ F.Debyser Z.Arosio D.Cereseto A.;
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作者单位

Laboratory of Neurobiology, Scuola Normale Superiore, 56124 Pisa, Italy;

Unité de Régulation des Infections Retrovirales, Institut Pasteur, 75724 Paris, France;

Laboratory of Molecular Virology and Gene Therapy, KU Leuven, 3000 Leuven, Flanders, BelgiumIstituto di Biofisica, Consiglio Nazionale Delle Ricerche, 38123 Trento, ItalyLaboratory of Molecular Virology, Centre for Integrative Biology, 38123 Trento, Italy;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
CBX-LEDGF; Retargeting; yH2AX;

Single-Cell Imaging of HIV-1 Provirus (SCIP)

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