Molecular assessment of surgical-resection margins of gastric cancer by mass-spectrometric imaging

Livia S. Eberlin; Robert J. Tibshirani; Jialing ZhangTeri A. LongacreGerald J. BerryDavid B. BinghamJeffrey A. NortonRichard N. ZareGeorge A. Poultsides

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Molecular assessment of surgical-resection margins of gastric cancer by mass-spectrometric imaging

机译：Molecular assessment of surgical-resection margins of gastric cancer by mass-spectrometric imaging

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Surgical resection is the main curative option for gastrointestinal cancers. The extent of cancer resection is commonly assessed during surgery by pathologic evaluation of (frozen sections of) the tissue at the resected specimen margin(s) to verify whether cancer is present. We compare this method to an alternative procedure, desorption electrospray ionization mass spectrometric imaging (DESI-MSI), for 62 banked human cancerous and normal gastrictissue samples. In DESI-MSI, microdroplets strike the tissue sample, the resulting splash enters a mass spectrometer, and a statistical analysis, here, the Lasso method (which stands for least absolute shrinkage and selection operator and which is a multiclass logistic regression with L1 penalty), is applied to classify tissues based on the molecular information obtained directly from DESI-MSI. The methodology developed with 28 frozen training samples of clear histopathologic diagnosis showed an overall accuracy value of 98 for the 12,480 pixels evaluated in cross-validation (CV), and 97 when a completely independent set of samples was tested. By applying an additional spatial smoothing technique, the accuracy for both CV and the independent set of samples was 99 compared with histological diagnoses. To test our method for clinical use, we applied it to a total of 21 tissue-margin samples prospectively obtained from nine gastric-cancer patients. The results obtained suggest that DESI-MSI/Lasso may be valuable for routine intraoperative assessment of the specimen margins during gastriccancer surgery.

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《Proceedings of the National Academy of Sciences of the United States of America》 |2014年第7期|2436-2441|共6页
作者
Livia S. Eberlin; Robert J. Tibshirani; Jialing ZhangTeri A. LongacreGerald J. BerryDavid B. BinghamJeffrey A. NortonRichard N. ZareGeorge A. Poultsides;
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作者单位

Department of Chemistry, Stanford University, Stanford, CA 94305-5080;

Departments of Health Research and Policy and of Statistics, Stanford University, Stanford, CA 94305-4065;

Department of Pathology, Stanford University, Stanford, CA 94305-5324Department of Surgery, Stanford University, Stanford, CA 94305-5641;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
mass spectrometry imaging; biostatistics; surgical margins; intraoperative diagnosis;

Molecular assessment of surgical-resection margins of gastric cancer by mass-spectrometric imaging

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