Molecular crowding limits translation and cell growth

Klumpp S.; Scott M.; Pedersen S.Hwa T.

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Molecular crowding limits translation and cell growth

机译：Molecular crowding limits translation and cell growth

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Bacterial growth is crucially dependent on protein synthesis and thus on the cellular abundance of ribosomes and related proteins. Here, we show that the slow diffusion of the bulky tRNA complexes in the crowded cytoplasm imposes a physical limit on the speed of translation, which ultimately limits the rate of cell growth. To study the required allocation of ancillary translational proteins to alleviate the effect of molecular crowding, we develop a model for cell growth based on a coarse-grained partitioning of the proteome. We find that coregulation of ribosome- and tRNA-affiliated proteins is consistent with measured growth-rate dependencies and results in near-optimal allocation over a broad range of growth rates. The analysis further resolves a long-standing controversy in bacterial growth physiology concerning the growth-rate dependence of translation speed and serves as a caution against premature identification of phenomenological parameters with mechanistic processes.

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《Proceedings of the National Academy of Sciences of the United States of America》 |2013年第42期|16754-16759|共6页
作者
Klumpp S.; Scott M.; Pedersen S.Hwa T.;
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作者单位

Department of Biology, University of Copenhagen, 2200 Copenhagen N, Denmark;

Department of Physics, Center for Theoretical Biological Physics, United States;

Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, GermanyDepartment of Applied Mathematics, University of Waterloo, Waterloo, ON, N2L 3G1, Canada;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
Molecular; crowding; limits;

Molecular crowding limits translation and cell growth

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