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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Amylopectin aggregation as a function of starch phosphate content studied by size exclusion chromatography and on-line refractive index and light scattering
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Amylopectin aggregation as a function of starch phosphate content studied by size exclusion chromatography and on-line refractive index and light scattering

机译：通过尺寸排阻色谱法，在线折射率和光散射研究支链淀粉的聚集与淀粉磷酸酯含量的关系

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摘要

Starches with a natural 65-fold span in covalently bound phosphate content were prepared from five different crops including sorghum, cassava, three potato varieties and an exotic ginger plant, Curcuma zedoaria, with extreme starch phosphate content. These starches were subjected to size exclusion chromatography with refractive index detection (SEC/RI). A simple and rapid method for starch solubilisation was used. The conditions during solubilisation (2 M NaOH) and separation (10 mM NaOH, 50 degreesC) were such as enabling > 94% recovery of the starch without detectable degradation. The aggregation properties of the starch was investigated using on line refractive index/multi angle laser light scattering (RI/MALLS) detection. Three major regions in the SEC profile were identified, consisting of large amylopectin aggregates, amylopectin particles with radius of gyration (R-g) of approx 200 nm (400 nm blocklets) and amylose. A procedure for correction of light scattering signals spread over the SEC profile as a result of aggregate tailing was developed. The significance of the relative amounts of these three molecular species on standard starch pasting parameters, as measured by a Rapid Visco Analyzer (RVA), was investigated. Starches with a high amount of amylopectin aggregates showed high peak viscosities. Moreover, very high amounts of starch bound phosphate or amylose appears to suppress the content of large aggregates resulting in low viscosity. (C) 2001 Elsevier Science B.V. All rights reserved. [References: 58]
机译：从五种不同的作物中制得了具有65倍天然天然的共价结合磷酸盐含量的淀粉，这些作物包括高粱，木薯，三种马铃薯品种和一种外来生姜植物姜黄（Cercuma zedoaria），其中淀粉的淀粉含量极高。这些淀粉经过具有折光率检测（SEC / RI）的尺寸排阻色谱法。使用了一种简单而快速的淀粉增溶方法。增溶（2 M NaOH）和分离（10 mM NaOH，50℃）过程中的条件使淀粉的回收率> 94％而无可检测的降解。使用在线折射率/多角度激光散射（RI / MALLS）检测研究淀粉的聚集特性。确定了SEC轮廓中的三个主要区域，包括大的支链淀粉聚集体，具有大约200 nm（400 nm小块）的回转半径（R-g）的支链淀粉和直链淀粉。开发了一种校正由于聚集拖尾而散布在SEC轮廓上的光散射信号的程序。研究了这三种分子的相对量对标准淀粉糊化参数的显着性，如通过快速粘度分析仪（RVA）测得的。支链淀粉含量高的淀粉具有较高的峰值粘度。而且，非常大量的淀粉结合的磷酸酯或直链淀粉似乎抑制大聚集体的含量，导致低粘度。 （C）2001 Elsevier Science B.V.保留所有权利。 [参考：58]

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