Water-soluble chlorophyll protein is involved in herbivore resistance activation during greening of Arabidopsis thaliana

Boex-Fontvieille Edouard; Rustgi Sachin; von Wettstein DiterReinbothe SteffenReinbothe Christiane

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Water-soluble chlorophyll protein is involved in herbivore resistance activation during greening of Arabidopsis thaliana

机译：Water-soluble chlorophyll protein is involved in herbivore resistance activation during greening of Arabidopsis thaliana

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Water-soluble chlorophyll proteins (WSCPs) constitute a small family of unusual chlorophyll (Chl)-binding proteins that possess a Kunitz-type protease inhibitor domain. In Arabidopsis thaliana, a WSCP has been identified, named AtWSCP, that forms complexes with Chl and the Chl precursor chlorophyllide (Chlide) in vitro. AtWSCP exhibits a quite unexpected expression pattern for a Chl binding protein and accumulated to high levels in the apical hook of etiolated plants. AtWSCP expression was negatively light-regulated. Transgenic expression of AtWSCP fused to green fluorescent protein (GFP) revealed that AtWSCP is localized to cell walls/apoplastic spaces. Biochemical assays identified AtWSCP as interacting with RD21 (RESPONSIVE TO DESICCATION 21), a granulin domain-containing cysteine protease implicated in stress responses and defense. Reconstitution experiments showed tight interactions between RD21 and WSCP that were relieved upon Chlide binding. Laboratory feeding experiments with two herbivorous isopod crustaceans, Porcellio scaber (woodlouse) and Armadillidium vulgare (pillbug), identified the apical hook as Achilles' heel of etiolated plants and that this was protected by RD21 during greening. Because Chlide is formed in the apical hook during seedling emergence from the soil, our data suggest an unprecedented mechanism of herbivore resistance activation that is triggered by light and involves AtWSCP.

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《Proceedings of the National Academy of Sciences of the United States of America》 |2015年第23期|7303-7308|共6页
作者
Boex-Fontvieille Edouard; Rustgi Sachin; von Wettstein DiterReinbothe SteffenReinbothe Christiane;
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作者单位

Univ Grenoble 1, Lab Genet Mol Plantes {L-End}& Biol Environm {L-End}& Syst, Lab Bioenerget Fondamentale {L-End}& Appl, F-38041 Grenoble 9, France;

Washington State Univ, Dept Crop {L-End}& Soil Sci, Mol Plant Sci, Sch Mol Biosci, Pullman, WA 99164 USA;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
skotomorphogenesis; plant defense; herbivore deterrence; cysteine proteases; kunitz protease inhibitor;

Water-soluble chlorophyll protein is involved in herbivore resistance activation during greening of Arabidopsis thaliana

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