Ribosome Protein L4 is essential for Epstein-Barr Virus Nuclear Antigen 1 function

Shen Chih-Lung; Liu Cheng-Der; You Ren-InChing Yung-HaoLiang JunKe LiangruChen Ya-LinChen Hong-ChiHsu Hao-JenLiou Je-WenKieff ElliottPeng Chih-Wen

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Ribosome Protein L4 is essential for Epstein-Barr Virus Nuclear Antigen 1 function

机译：Ribosome Protein L4 is essential for Epstein-Barr Virus Nuclear Antigen 1 function

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Epstein-Barr Virus (EBV) Nuclear Antigen 1 (EBNA1)-mediated origin of plasmid replication (oriP) DNA episome maintenance is essential for EBV-mediated tumorigenesis. We have now found that EBNA1 binds to Ribosome Protein L4 (RPL4). RPL4 shRNA knockdown decreased EBNA1 activation of an oriP luciferase reporter, EBNA1 DNA binding in lymphoblastoid cell lines, and EBV genome number per lymphoblastoid cell line. EBV infection increased RPL4 expression and redistributed RPL4 to cell nuclei. RPL4 and Nucleolin (NCL) were a scaffold for an EBNA1-induced oriP complex. The RPL4 N terminus cooperated with NCL-K429 to support EBNA1 and oriP-mediated episome binding and maintenance, whereas the NCL C-terminal K380 and K393 induced oriP DNA H3K4me2 modification and promoted EBNA1 activation of oriP-dependent transcription. These observations provide new insights into the mechanisms by which EBV uses NCL and RPL4 to establish persistent B-lymphoblastoid cell infection.

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《Proceedings of the National Academy of Sciences of the United States of America.》 |2016年第8期|2229-2234|共6页
作者
Shen Chih-Lung; Liu Cheng-Der; You Ren-InChing Yung-HaoLiang JunKe LiangruChen Ya-LinChen Hong-ChiHsu Hao-JenLiou Je-WenKieff ElliottPeng Chih-Wen;
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作者单位

Tzu Chi Univ, Inst Med Sci, Hualien 97004, Taiwan;

Tzu Chi Univ, Dept Lab Med & Biotechnol, Sec 3, Hualien 97004, Taiwan;

Harvard Univ, Sch Med, Dept Microbiol & Immunobiol, Boston, MA 02115 USATzu Chi Univ, Dept Life Sci, Sec 3, Hualien 97004, TaiwanTzu Chi Univ, Dept Mol Biol & Human Genet, Sec 3, Hualien 97004, TaiwanTzu Chi Univ, Inst Biochem Sci, Sec 3, Hualien 97004, Taiwan;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
EBNA1; RPL4; NCL; EBV; oriP;

Ribosome Protein L4 is essential for Epstein-Barr Virus Nuclear Antigen 1 function

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