Using 3-mercaptopropyltrimethoxysilane (MPS)-coated (PbMg_(1/3)Nb_(2/3)O_(3))_(0.63)-(PbTiO_(3))_(0.37) (PMN-PT)/tin and lead zirconate titanate/glass piezoelectric microcantilever sensors (PEMSs) with single-chain variable fragment (scFv) immobilized on the MPS surface, we have demonstrated real-time, label-free detection of human epidermal growth factor receptor 2 (Her2) in a background of 1 mg/ml bovine serum albumin. Coupled with a scFv with a K_(D) of 3.4 X 10~(-8)M, the MPS-insulated PMN-PT/tin PEMS 560 (mu)m long and 720 (mu)m wide exhibited a Her2 concentration sensitivity of 5 ng/ml in a background of 1 mg/ml BSA.
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