Sample holder for small-angle x-ray scattering static and flow cell measurements

Lipfert  J; Millett  IS; Seifert  SDoniach  S

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Sample holder for small-angle x-ray scattering static and flow cell measurements

机译：Sample holder for small-angle x-ray scattering static and flow cell measurements

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摘要

We present the design of a sample holder for small-angle x-ray scattering (SAXS) that can be used for both static and flow cell measurements, allowing to switch between these two types of measurement without having to realign the detector and camera geometry. The device makes possible high signal-to-noise experiments with sample volumes as small as 16 mu l and can be thermocontrolled using a standard circulating water bath. The setup has been used successfully for a range of biological SAXS measurements, including peptides, detergent micelles, membrane proteins, and nucleic acids. As a performance test, we present scattering data for horse heart cytochrome c, collected at the BESSRC CAT beam line 12-ID of the Advanced Photon Source. The design drawings are provided in the supplementary material. (c) 2006 American Institute of Physics.

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《Review of Scientific Instruments》 |2006年第4期|46108-1-46108-3-0|共3页
作者
Lipfert J; Millett IS; Seifert SDoniach S;
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作者单位

Stanford Univ, Geballe Lab Adv Mat, Dept Phys, Stanford, CA 94305 USA;

Stanford Univ, Geballe Lab Adv Mat, Dept Appl Phys, Stanford, CA 94305 USA;

Stanford Univ, Geballe Lab Adv Mat, Biophys Program, Stanford, CA 94305 USAArgonne Natl Lab, Expt Facil Div, Argonne, IL 60439 USA;

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原文格式 PDF
正文语种英语
中图分类一般性问题;
关键词
ADVANCED PHOTON SOURCE; BIOLOGICAL MACROMOLECULES; CYTOCHROME-C;

Sample holder for small-angle x-ray scattering static and flow cell measurements

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