Cas9-mediated targeting of viral RNA in eukaryotic cells

Price Aryn A.; Sampson Timothy R.; Ratner Hannah K.Grakoui ArashWeiss David S.

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Cas9-mediated targeting of viral RNA in eukaryotic cells

机译：Cas9-mediated targeting of viral RNA in eukaryotic cells

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Clustered, regularly interspaced, short palindromic repeats-CRISPR associated (CRISPR-Cas) systems are prokaryotic RNA-directed endonuclease machineries that act as an adaptive immune system against foreign genetic elements. Using small CRISPR RNAs that provide specificity, Cas proteins recognize and degrade nucleic acids. Our previous work demonstrated that the Cas9 endonuclease from Francisella novicida (FnCas9) is capable of targeting endogenous bacterial RNA. Here, we show that FnCas9 can be directed by an engineered RNA-targeting guide RNA to target and inhibit a human +ssRNA virus, hepatitis C virus, within eukaryotic cells. This work reveals a versatile and portable RNA-targeting system that can effectively function in eukaryotic cells and be programmed as an antiviral defense.

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《Proceedings of the National Academy of Sciences of the United States of America》 |2015年第19期|6164-6169|共6页
作者
Price Aryn A.; Sampson Timothy R.; Ratner Hannah K.Grakoui ArashWeiss David S.;
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作者单位

Emory Univ, Microbiol & Mol Genet Program, Dept Microbiol & Immunol, Atlanta, GA 30329 USA;

Emory Univ, Emory Vaccine Ctr, Atlanta, GA 30329 USA;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
Cas9; CRISPR-Cas; hepatitis C virus; RNA targeting; Francisella;

Cas9-mediated targeting of viral RNA in eukaryotic cells

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