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首页> 外文期刊>Review of Scientific Instruments >Self-contained high-pressure cell, apparatus, and procedure for the preparation of encapsulated proteins dissolved in low viscosity fluids for nuclear magnetic resonance spectroscopy
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Self-contained high-pressure cell, apparatus, and procedure for the preparation of encapsulated proteins dissolved in low viscosity fluids for nuclear magnetic resonance spectroscopy

机译：Self-contained high-pressure cell, apparatus, and procedure for the preparation of encapsulated proteins dissolved in low viscosity fluids for nuclear magnetic resonance spectroscopy

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摘要

The design of a sample cell for high-performance nuclear magnetic resonance (NMR) at elevated pressure is described. The cell has been optimized for the study of encapsulated proteins dissolved in low viscosity fluids but is suitable for more general nuclear magnetic resonance (NMR) spectroscopy of biomolecules at elevated pressure. The NMR cell is comprised of an alumina-toughened zirconia tube mounted on a self-sealing nonmagnetic metallic valve. The cell has several advantages, including relatively low cost, excellent NMR performance, high-pressure tolerance, chemical inertness, and a relatively large active volume. Also described is a low volume sample preparation device that allows for the preparation of samples under high hydrostatic pressure and their subsequent transfer to the NMR cell.

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