Antibodies with infinite affinity.

Chmura AJ; Orton MS; Meares CF

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Antibodies with infinite affinity.

机译：Antibodies with infinite affinity.

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Here we report an approach to the design and production of antibody/ligand pairs, to achieve functional affinity far greater than avidin/biotin. Using fundamental chemical principles, we have developed antibody/ligand pairs that retain the binding specificity of the antibody, but do not dissociate. Choosing a structurally characterized antibody/ligand pair as an example, we engineered complementary reactive groups in the antibody binding pocket and the ligand, so that they would be in close proximity in the antibody/ligand complex. Cross-reactions with other molecules in the medium are averted because of the low reactivity of these groups; however, in the antibody/ligand complex the effective local concentrations of the complementary reactive groups are very large, allowing a covalent reaction to link the two together. By eliminating the dissociation of the ligand from the antibody, we have made the affinity functionally infinite. This chemical manipulation of affinity is applicable to other biological binding pairs.

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《Proceedings of the National Academy of Sciences of the United States of America》 |2001年第15期|8480-8484|共5页
作者
Chmura AJ; Orton MS; Meares CF;
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作者单位

Department of Chemistry, University of California, One Shields Avenue, Davis, CA 95616, USA.;

hylo.life.uiuc.edu;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
Antibodies; Monoclonal; Antibody Affinity; Chelating Agents; Edetic Acid; 抗体; 单克隆; 抗体亲和力; 螯合剂; 依地酸; 免疫球蛋白类; Fab; 破伤风类毒素;

Antibodies with infinite affinity.

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