ABSTRACTAspergillus parasiticusBCRI, a caffeine‐resistant mutant of A.parasiticusNRRL 2999, produced abundant amounts of aflatoxins (AF) in yeast extract‐sucrose broth only when the medium was supplemented with caffeine. However, little AF production occurred in glucose‐mineral salts medium (GMS) regardless of the level of caffeine supplementation. Caffeine‐dependent AF production was restored if GMS were fortified with peptone or some other source of amino acids. Subsequent studies indicated that this effect could be achieved by supplementing GMS with specific amino acids, particularly proline, alanine, methionine, arginine or asparagine. Restoration of caffeine‐dependent AF synthesis did not occur when GMS was supplemented with purine bases or nucleotides. The results indicated that caffeine‐dependent AF production in BCRI was dependent on amino acid
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