Dynamic regulation of eve stripe 2 expression reveals transcriptional bursts in living Drosophila embryos

Jacques P. Bothma; Hernan G. Garcia; Emilia EspositoGavin SchlisselThomas GregorMichael Levine

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Dynamic regulation of eve stripe 2 expression reveals transcriptional bursts in living Drosophila embryos

机译：Dynamic regulation of eve stripe 2 expression reveals transcriptional bursts in living Drosophila embryos

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We present the use of recently developed live imaging methods to examine the dynamic regulation of even-skipped (eve) stripe 2 expression in the precellular Drosophila embryo. Nascent transcripts were visualized via MS2 RNA stem loops. The eve stripe 2 transgene exhibits a highly dynamic pattern of de novo transcription, beginning with a broad domain of expression during nuclear cycle 12 (nc12), and progressive refinement during nc13 and nc14. The mature stripe 2 pattern is surprisingly transient, constituting just ～15 min of the ～90-min period of expression. Nonetheless, this dynamic transcription profile faithfully predicts the limits of the mature stripe visualized by conventional in situ detection methods. Analysis of individual transcription foci reveals intermittent bursts of de novo transcription, with duration cycles of 4–10 min. We discuss a multistate model of transcription regulation and speculate on its role in the dynamic repression of the eve stripe 2 expression pattern during development.

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《Proceedings of the National Academy of Sciences of the United States of America》 |2014年第29期|10598-10603|共6页
作者
Jacques P. Bothma; Hernan G. Garcia; Emilia EspositoGavin SchlisselThomas GregorMichael Levine;
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作者单位

Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720;

Joseph Henry Laboratories of Physics, Princeton University, Princeton, NJ 08544;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
regulation; expression; embryos;

Dynamic regulation of eve stripe 2 expression reveals transcriptional bursts in living Drosophila embryos

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