Transport efficiency of membrane-anchored kinesin-1 motors depends on motor density and diffusivity

Grover Rahul; Fischer Janine; Schwarz Friedrich W.Walter Wilhelm J.Schwille PetraDiez Stefan

首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America. >Transport efficiency of membrane-anchored kinesin-1 motors depends on motor density and diffusivity

【24h】

Transport efficiency of membrane-anchored kinesin-1 motors depends on motor density and diffusivity

机译：Transport efficiency of membrane-anchored kinesin-1 motors depends on motor density and diffusivity

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

In eukaryotic cells, membranous vesicles and organelles are transported by ensembles of motor proteins. These motors, such as kinesin-1, have been well characterized in vitro as single molecules or as ensembles rigidly attached to nonbiological substrates. However, the collective transport by membrane-anchored motors, that is, motors attached to a fluid lipid bilayer, is poorly understood. Here, we investigate the influence of motors' anchorage to a lipid bilayer on the collective transport characteristics. We reconstituted "membrane-anchored" gliding motility assays using truncated kinesin-1 motors with a streptavidin-binding peptide tag that can attach to streptavidin-loaded, supported lipid bilayers. We found that the diffusing kinesin-1 motors propelled the microtubules in the presence of ATP. Notably, we found the gliding velocity of the microtubules to be strongly dependent on the number of motors and their diffusivity in the lipid bilayer. The microtubule gliding velocity increased with increasing motor density and membrane viscosity, reaching up to the stepping velocity of single motors. This finding is in contrast to conventional gliding motility assays where the density of surface-immobilized kinesin-1 motors does not influence the microtubule velocity over a wide range. We reason that the transport efficiency of membrane-anchored motors is reduced because of their slippage in the lipid bilayer, an effect that we directly observed using single-molecule fluorescence microscopy. Our results illustrate the importance of motor-cargo coupling, which potentially provides cells with an additional means of regulating the efficiency of cargo transport.

著录项

来源
《Proceedings of the National Academy of Sciences of the United States of America.》 |2016年第46期|E7185-E7193|共9页
作者
Grover Rahul; Fischer Janine; Schwarz Friedrich W.Walter Wilhelm J.Schwille PetraDiez Stefan;
展开▼
作者单位

Tech Univ Dresden, Ctr Mol Bioengn B CUBE, D-01069 Dresden, Germany;

Max Planck Inst Mol Cell Biol & Genet, D-01307 Dresden, Germany;

Max Planck Inst Biochem, Dept Cellular & Mol Biophys, D-82152 Martinsried, Germany;

展开▼
收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
molecular motors; lipid bilayers; transport efficiency; motor-cargo coupling; streptavidin-binding peptide;

Transport efficiency of membrane-anchored kinesin-1 motors depends on motor density and diffusivity

摘要

著录项

相关主题

期刊订阅