Positive regulation by small RNAs and the role of Hfq

Soper T.; Mandin P.; Majdalani N.Gottesman S.Woodson S.A.

首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Positive regulation by small RNAs and the role of Hfq

【24h】

Positive regulation by small RNAs and the role of Hfq

机译：Positive regulation by small RNAs and the role of Hfq

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

Bacterial small noncoding RNAs carry out both positive and negative. Escherichia coli, this regulation often requires the RNA chaperone Hfq. Three small regulatory RNAs (sRNAs), DsrA, RprA, and ArcZ, positively regulate translation of the sigma factor RpoS, each pairing with the 5′ leader to open up an inhibitory hairpin. In vitro, rpoS interaction with sRNAs depends upon an (AAN)_4 Hfq-binding site upstream of the pairing region. Here we show that both Hfq and this Hfq binding site are required for RprA or ArcZ to act in vivo and to form a stable complex with rpoS mRNA in vitro; both were partially dispensable for DsrA at 37°C. ArcZ sRNA is processed from 121 nt to a stable 56 nt species that contains the pairing region; only the 56 nt ArcZ makes a strong Hfq-dependent complex with rpoS. For each of these sRNAs, the stability of the sRNA ? mRNA complexes, rather than their rate of formation, best predicted in vivo activity. These studies demonstrate that binding of Hfq to the rpoS mRNA is critical for sRNA regulation under normal conditions, but if the stability of the sRNA ? mRNA complex is sufficiently high, the requirement for Hfq can be bypassed.

著录项

来源
《Proceedings of the National Academy of Sciences of the United States of America》 |2010年第21期|9602-9607|共6页
作者
Soper T.; Mandin P.; Majdalani N.Gottesman S.Woodson S.A.;
展开▼
作者单位

Johns Hopkins University, Program in Cell, Molecular, Developmental Biology and Biophysics, 3400 North Charles Street, Baltimore, MD 21218, United States;

Laboratory of Molecular Biology, Building 37, National Cancer Institute, Bethesda, MD 20892, United States;

展开▼
收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
RNA-protein interactions; Sigma 38; Sm-like protein; Translational control;

Positive regulation by small RNAs and the role of Hfq

摘要

著录项

相关主题

期刊订阅