DEAD-box protein CYT-19 is activated by exposed helices in a group I intron RNA

Inga Jarmoskaite; Hari Bhaskaran; Soenke SeifertRick Russell

首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >DEAD-box protein CYT-19 is activated by exposed helices in a group I intron RNA

【24h】

DEAD-box protein CYT-19 is activated by exposed helices in a group I intron RNA

机译：DEAD-box protein CYT-19 is activated by exposed helices in a group I intron RNA

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

DEAD-box proteins are nonprocessive RNA helicases and can function as RNA chaperones, but the mechanisms of their chaperone activity remain incompletely understood. The Neurospora crassa DEAD-box protein CYT-19 is a mitochondrial RNA chaperone that promotes group I intron splicing and has been shown to resolve misfolded group I intron structures, allowing them to refold. Building on previous results, here we use a series of tertiary contact mutants of the Tetrahymena group I intron ribozyme to demonstrate that the efficiency of CYT-19–mediated unfolding of the ribozyme is tightly linked to global RNA tertiary stability. Efficient unfolding of destabilized ribozyme variants is accompanied by increased ATPase activity of CYT-19, suggesting that destabilized ribozymes provide more productive interaction opportunities. The strongest ATPase stimulation occurs with a ribozyme that lacks all five tertiary contacts and does not form a compact structure, and small-angle X-ray scattering indicates that ATPase activity tracks with ribozyme compactness. Further, deletion of three helices that are prominently exposed in the folded structure decreases the ATPase stimulation by the folded ribozyme. Together, these results lead to a model in which CYT-19, and likely related DEAD-box proteins, rearranges complex RNA structures by preferentially interacting with and unwinding exposed RNA secondary structure. Importantly, this mechanism could bias DEAD-box proteins to act on misfolded RNAs and ribonucleoproteins, which are likely to be less compact and more dynamic than their native counterparts.

著录项

来源
《Proceedings of the National Academy of Sciences of the United States of America》 |2014年第29期|E2928-E2936|共9页
作者
Inga Jarmoskaite; Hari Bhaskaran; Soenke SeifertRick Russell;
展开▼
作者单位

Department of Molecular Biosciences, University of Texas at Austin, Austin, TX 78712;

X-Ray Science Division, Advanced Photon Source, Argonne National Laboratory, Argonne, IL 60439;

展开▼
收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
RNA folding; RNA misfolding; RNA tertiary structure; RNA unwinding; superfamily 2 helicase;

DEAD-box protein CYT-19 is activated by exposed helices in a group I intron RNA

摘要

著录项

相关主题

期刊订阅