TRNA tK~(UUU), tQ~(UUG), and tE~(UUC) wobble position modifications fine-tune protein translation by promoting ribosome A-site binding

Rezgui V.A.N.; Tyagi K.; Ranjan N.Konevega A.L.Mittelstaet J.Rodnina M.V.Peter M.Pedrioli P.G.A.

首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >TRNA tK~(UUU), tQ~(UUG), and tE~(UUC) wobble position modifications fine-tune protein translation by promoting ribosome A-site binding

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TRNA tK~(UUU), tQ~(UUG), and tE~(UUC) wobble position modifications fine-tune protein translation by promoting ribosome A-site binding

机译：TRNA tK~(UUU), tQ~(UUG), and tE~(UUC) wobble position modifications fine-tune protein translation by promoting ribosome A-site binding

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tRNA modifications are crucial to ensure translation efficiency and fidelity. In eukaryotes, the URM1 and ELP pathways increase cellular resistance to various stress conditions, such as nutrient starvation and oxidative agents, by promoting thiolation and methoxycarbonylmethylation, respectively, of the wobble uridine of cytoplasmic tRNAUUU Lys (tK~(UUU)), tRNAUUG Gln (tQ~(UUG)), and tRNAUUC Glu (tE~(UUC)). Although in vitro experiments have implicated these tRNA modifications in modulating wobbling capacity and translation efficiency, their exact in vivo biological roles remain largely unexplored. Using a combination of quantitative proteomics and codon- specific translation reporters, we find that translation of a specific gene subset enriched for AAA, CAA, and GAA codons is impaired in the absence of URM1- and ELP-dependent tRNA modifications. Moreover, in vitro experiments using native tRNAs demonstrate that both modifications enhance binding of tK~(UUU) to the ribosomal A-site. Taken together, our data suggest that tRNA thiolation and methoxycarbonylmethylation regulate translation of genes with specific codon content.

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《Proceedings of the National Academy of Sciences of the United States of America》 |2013年第30期|12289-12294|共6页
作者
Rezgui V.A.N.; Tyagi K.; Ranjan N.Konevega A.L.Mittelstaet J.Rodnina M.V.Peter M.Pedrioli P.G.A.;
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作者单位

Department of Physical Biochemistry, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany;

Department of Biology, Institute of Biochemistry, Eidgen?ssiche Technische Hochschule Zürich, 8093 Zurich, Switzerland;

Scottish Institute for Cell Signalling, College of Life Sciences, University of Dundee, DD1 5EH Dundee, United Kingdom;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
SILAC; Systems biology; Translation regulation;

TRNA tK~(UUU), tQ~(UUG), and tE~(UUC) wobble position modifications fine-tune protein translation by promoting ribosome A-site binding

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