Nonredox thiolation in tRNA occurring via sulfur activation by a 4Fe-4S cluster

Arragain Simon; Bimai Ornella; Legrand PierreCaillat SylvainRavanat Jean-LucTouati NadiaBinet LaurentAtta MohamedFontecave MarcGolinelli-Pimpaneau Beatrice

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Nonredox thiolation in tRNA occurring via sulfur activation by a 4Fe-4S cluster

机译：Nonredox thiolation in tRNA occurring via sulfur activation by a 4Fe-4S cluster

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摘要

Sulfur is present in several nucleosides within tRNAs. In particular, thiolation of the universally conserved methyl-uridine at position 54 stabilizes tRNAs from thermophilic bacteria and hyperthermophilic archaea and is required for growth at high temperature. The simple nonredox substitution of the C2-uridine carbonyl oxygen by sulfur is catalyzed by tRNA thiouridine synthetases called TtuA. Spectroscopic, enzymatic, and structural studies indicate that TtuA carries a catalytically essential 4Fe-4S cluster and requires ATP for activity. A series of crystal structures shows that (i) the cluster is ligated by only three cysteines that are fully conserved, allowing the fourth unique iron to bind a small ligand, such as exogenous sulfide, and (ii) the ATP binding site, localized thanks to a protein-bound AMP molecule, a reaction product, is adjacent to the cluster. A mechanism for tRNA sulfuration is suggested, in which the unique iron of the catalytic cluster serves to bind exogenous sulfide, thus acting as a sulfur carrier.

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《Proceedings of the National Academy of Sciences of the United States of America.》 |2017年第28期|7355-7360|共6页
作者
Arragain Simon; Bimai Ornella; Legrand PierreCaillat SylvainRavanat Jean-LucTouati NadiaBinet LaurentAtta MohamedFontecave MarcGolinelli-Pimpaneau Beatrice;
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作者单位

Univ Paris 06, Lab Chim Proc Biol, UMR 8229, CNRS,Coll France, F-75231 Paris 05, France;

SOLEIL Synchrotron, F-91198 Gif Sur Yvette, France;

Univ Grenoble Alpes, Commissariat Energie Atom, Inst Nanosci & Cryogenie Syst Mol & Nanomat Energ, F-38054 Grenoble, FranceChim ParisTech, Inst Rech Renard, CNRS, F-75005 Paris, FranceUniv Grenoble Alpes, Inst Biosci & Biotechnol Grenoble, Commissariata Energie Atom,, Lab Chim & Biol Met,UMR 5249,Direct Rech Fondamen, F-38000 Grenoble, France;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
tRNA modification; thiolation; Fe-S cluster; thiouridine synthetase; U54-tRNA;

Nonredox thiolation in tRNA occurring via sulfur activation by a 4Fe-4S cluster

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