Numerous proteins with unique characteristics are degraded by the 26S proteasome following monoubiquitination

Braten Ori; Livneh Ido; Ziv TamarAdmon ArieKehat IzhakCaspi Lilac H.Gonen HedvaBercovich BeatriceGodzik AdamJahandideh SamadJaroszewski LukaszSommer ThomasKwon Yong TaeGuharoy MainakTompa PeterCiechanover Aaron

首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America. >Numerous proteins with unique characteristics are degraded by the 26S proteasome following monoubiquitination

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Numerous proteins with unique characteristics are degraded by the 26S proteasome following monoubiquitination

机译：Numerous proteins with unique characteristics are degraded by the 26S proteasome following monoubiquitination

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The "canonical" proteasomal degradation signal is a substrate-anchored polyubiquitin chain. However, a handful of proteins were shown to be targeted following monoubiquitination. In this study, we established-in both human and yeast cells-a systematic approach for the identification of monoubiquitination-dependent proteasomal substrates. The cellular wild-type polymerizable ubiquitin was replaced with ubiquitin that cannot form chains. Using proteomic analysis, we screened for substrates that are nevertheless degraded under these conditions compared with those that are stabilized, and therefore require polyubiquitination for their degradation. For randomly sampled representative substrates, we confirmed that their cellular stability is in agreement with our screening prediction. Importantly, the two groups display unique features: monoubiquitinated substrates are smaller than the polyubiquitinated ones, are enriched in specific pathways, and, in humans, are structurally less disordered. We suggest that monoubiquitination-dependent degradation is more widespread than assumed previously, and plays key roles in various cellular processes.

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《Proceedings of the National Academy of Sciences of the United States of America.》 |2016年第32期|E4639-E4647|共9页
作者
Braten Ori; Livneh Ido; Ziv TamarAdmon ArieKehat IzhakCaspi Lilac H.Gonen HedvaBercovich BeatriceGodzik AdamJahandideh SamadJaroszewski LukaszSommer ThomasKwon Yong TaeGuharoy MainakTompa PeterCiechanover Aaron;
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作者单位

Sanford Burnham Prebys Med Discovery Inst, Bioinformat & Syst Biol Program, La Jolla, CA 92037 USA;

Max Delbruck Ctr Mol Med, D-13125 Berlin, Germany;

Seoul Natl Univ, Coll Med, Prot Metab Med Res Ctr, Seoul 110799, South KoreaTechnion Israel Inst Technol, Rappaport Fac Med & Res Inst, Technion Integrated Canc Ctr, IL-3109602 Haifa, IsraelVrije Univ Brussel, Res Ctr, Vlaams Inst Biotechnol Struct Biol, B-1050 Brussels, BelgiumTechnion Israel Inst Technol, Smoler Prote Ctr, IL-3200003 Haifa, IsraelTechnion Israel Inst Technol, Rappaport Fac Med & Res Inst, Dept Physiol, IL-3109602 Haifa, Israel;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
monoubiquitination; 26S proteasome; protein degradation; ubiquitin replacement;

Numerous proteins with unique characteristics are degraded by the 26S proteasome following monoubiquitination

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