Design and characterization of synthetic fungal-bacterial consortia for direct production of isobutanol from cellulosic biomass

Minty J.J.; Singer M.E.; Scholz S.A.Bae C.-H.Ahn J.-H.Foster C.E.Liao J.C.Lin X.N.

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Design and characterization of synthetic fungal-bacterial consortia for direct production of isobutanol from cellulosic biomass

机译：Design and characterization of synthetic fungal-bacterial consortia for direct production of isobutanol from cellulosic biomass

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Synergistic microbial communities are ubiquitous in nature and exhibit appealing features, such as sophisticated metabolic capabilities and robustness. This has inspired fast-growing interest in engineering synthetic microbial consortia for biotechnology development. However, there are relatively few reports of their use in real-world applications, and achieving population stability and regulation has proven to be challenging. In this work, we bridge ecology theory with engineering principles to develop robust synthetic fungal-bacterial consortia for efficient biosynthesis of valuable products from lignocellulosic feedstocks. The required biological functions are divided between two specialists: the fungus Trichoderma reesei, which secretes cellulase enzymes to hydrolyze lignocellulosic biomass into soluble saccharides, and the bacterium Escherichia coli, which metabolizes soluble saccharides into desired products. We developed and experimentally validated a comprehensive mathematical model for T. reesei/E. coli consortia, providing insights on key determinants of the system's performance. To illustrate the bioprocessing potential of this consortium, we demonstrate direct conversion of microcrystalline cellulose and pretreated corn stover to isobutanol. Without costly nutrient supplementation, we achieved titers up to 1.88 g/L and yields up to 62 of theoreticalmaximum. In addition,we show that cooperator-cheater dynamics within T. reesei/E. coli consortia lead to stable population equilibria and provide a mechanism for tuning composition. Although we offer isobutanol production as a proof-of-concept application, our modular system could be readily adapted for production of many other valuable biochemicals.

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《Proceedings of the National Academy of Sciences of the United States of America》 |2013年第36期|14592-14597|共6页
作者
Minty J.J.; Singer M.E.; Scholz S.A.Bae C.-H.Ahn J.-H.Foster C.E.Liao J.C.Lin X.N.;
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作者单位

Department of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109, United States;

Cellular and Molecular Biology Ph.D. Program, University of Michigan, Ann Arbor, MI 48109, United States;

Department of Energy Great Lakes Bioenergy Research Center, Michigan State University, Lansing, MI 48824, United StatesDepartment of Chemical and Biomolecular Engineering, University of California, Los Angeles, CA 90095, United States;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
Consolidated bioprocessing; Lignocellulosic biofuel; Renewable energy;

Design and characterization of synthetic fungal-bacterial consortia for direct production of isobutanol from cellulosic biomass

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