Plastidial transporters KEA1, -2, and -3 are essential for chloroplast osmoregulation, integrity, and pH regulation in Arabidopsis

Hans-Henning Kunz; Markus Gierth; Andrei HerdeanMio Satoh-CruzDavid M. KramerCornelia SpeteaJulian I. Schroeder

首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Plastidial transporters KEA1, -2, and -3 are essential for chloroplast osmoregulation, integrity, and pH regulation in Arabidopsis

【24h】

Plastidial transporters KEA1, -2, and -3 are essential for chloroplast osmoregulation, integrity, and pH regulation in Arabidopsis

机译：Plastidial transporters KEA1, -2, and -3 are essential for chloroplast osmoregulation, integrity, and pH regulation in Arabidopsis

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

Multiple K~+ transporters and channels and the corresponding mutants have been described and studied in the plasma membrane and organelle membranes of plant cells. However, knowledge about the molecular identity of chloroplast K~+ transporters is limited. Potassium transport and a well-balanced K~+ homeostasis were suggested to play important roles in chloroplast function. Because no loss-of-function mutants have been identified, the importance of K~+ transporters for chloroplast function and photosynthesis remains to be determined. Here, we report single and higherorder loss-of-function mutants in members of the cation/proton antiporters-2 antiporter superfamily KEA1, KEA2, and KEA3. KEA1 and KEA2 proteins are targeted to the inner envelope membrane of chloroplasts, whereas KEA3 is targeted to the thylakoid membrane. Higher-order but not single mutants showed increasingly impaired photosynthesis along with pale green leaves and severely stunted growth. The pH component of the proton motive force across the thylakoid membrane was significantly decreased in the kea1kea2 mutants, but increased in the kea3 mutant, indicating an altered chloroplast pH homeostasis. Electron microscopy of kea1kea2 leaf cells revealed dramatically swollen chloroplasts with disrupted envelope membranes and reduced thylakoid membrane density. Unexpectedly, exogenous NaCl application reversed the observed phenotypes. Furthermore, the kea1kea2 background enables genetic analyses of the functional significance of other chloroplast transporters as exemplified here in kea1kea2Na~+/H~+ antiporter1 (nhd1) triple mutants. Taken together, the presented data demonstrate a fundamental role of inner envelope KEA1 and KEA2 and thylakoid KEA3 transporters in chloroplast osmoregulation, integrity, and ion and pH homeostasis.

著录项

来源
《Proceedings of the National Academy of Sciences of the United States of America》 |2014年第20期|7480-7485|共6页
作者
Hans-Henning Kunz; Markus Gierth; Andrei HerdeanMio Satoh-CruzDavid M. KramerCornelia SpeteaJulian I. Schroeder;
展开▼
作者单位

Division of Biological Sciences, Section of Cell and Developmental Biology, Center for Food and Fuel for the 21st Century, University of California, San Diego, La Jolla, CA 92093-0116;

Department of Botany II, University of Cologne, 50674 Cologne, Germany;

Department of Biological and Environmental Sciences, University of Gothenburg, 40530 Gothenburg, SwedenDepartment of Energy Plant Research, Michigan State University, East Lansing, MI 48824-1312;

展开▼
收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
corresponding; plasma; molecular;

Plastidial transporters KEA1, -2, and -3 are essential for chloroplast osmoregulation, integrity, and pH regulation in Arabidopsis

摘要

著录项

相关主题

期刊订阅