microRNAs are biomarkers of oncogenic human papillomavirus infections

Xiaohong Wang; Hsu-Kun Wang; Yang LiMarkus HafnerNilam Sanjib BanerjeeShuang TangDaniel BriskinCraig MeyersLouise T. ChowXing XieThomas TuschlZhi-Ming Zheng

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microRNAs are biomarkers of oncogenic human papillomavirus infections

机译：microRNAs are biomarkers of oncogenic human papillomavirus infections

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Cellular and viral microRNAs (miRNAs) are the transcriptional products of RNA polymerase II and are regulated by transcriptional factors for their differential expression. The altered expression of miRNAs in many cancer types has been explored as a marker for possible diagnosis and therapy. We report in this study that oncogenic human papillomaviruses (HPVs) induce aberrant expression of many cellular miRNAs and that HPV18 infection produces no detectable viral miRNA. Thirteen abundant host miRNAs were specifically regulated by HPV16 and HPV18 in organotypic raft cultures of foreskin and vaginal keratinocytes as determined by miRNA array in combination with small RNA sequencing. The increase of miR-16, miR-25, miR-92a, and miR-378 and the decrease of miR-22, miR-27a, miR-29a, and miR-100 could be attributed to viral oncoprotein E6, E7, or both, all of which are known to target many cellular transcription factors. The examination of 158 cervical specimens, including 38 normal, 52 cervical intraepithelial neoplasia (CIN), and 68 cervical cancer (CC) tissues, for the expression of these eight miRNAs showed a remarkable increase of miR-25, miR-92a, and miR-378 with lesion progression but no obvious change of miR-22, miR-29a, and miR-100 among the HPV-infected tissues. Further analyses indicate that an expression ratio ≥1.5 of miR-25/92a group over miR-22/29a group could serve as a cutoff value to distinguish normal cervix from CIN and from CIN to CC.

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《Proceedings of the National Academy of Sciences of the United States of America》 |2014年第11期|4262-4267|共6页
作者
Xiaohong Wang; Hsu-Kun Wang; Yang LiMarkus HafnerNilam Sanjib BanerjeeShuang TangDaniel BriskinCraig MeyersLouise T. ChowXing XieThomas TuschlZhi-Ming Zheng;
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作者单位

Tumor Virus RNA Biology Section, Gene Regulation and Chromosome Biology Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892;

Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL 35294-0005;

Howard Hughes Medical Institute, Rockefeller University, New York, NY 10065-6399Department of Gynecologic Oncology, Women’s Hospital, Zhejiang University School of Medicine, Hangzhou 310006, ChinaDepartment of Microbiology and Immunology, Penn State University College of Medicine, Hershey, PA 17033;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
biomarkers; human; papillomavirus;

microRNAs are biomarkers of oncogenic human papillomavirus infections

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