X-ray snapshots of possible intermediates in the time course of synthesis and degradation of protein-bound Fe4S4 clusters

Nicolet Y.; Rohac R.; Martin L.Fontecilla-Camps J.C.

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X-ray snapshots of possible intermediates in the time course of synthesis and degradation of protein-bound Fe4S4 clusters

机译：X-ray snapshots of possible intermediates in the time course of synthesis and degradation of protein-bound Fe4S4 clusters

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Fe_4S_4 clusters are very common versatile prosthetic groups in proteins. Their redox property of being sensitive to O _2-induced oxidative damage is, for instance, used by the cell to sense oxygen levels and switch between aerobic and anaerobic metabolisms, as exemplified by the fumarate, nitrate reduction regulator (FNR). Using the hydrogenase maturase HydE from Thermotoga maritima as a template, we obtained several unusual forms of FeS clusters, some of which are associated with important structural changes. These structures represent intermediate states relevant to both FeS cluster assembly and degradation. We observe one Fe _2S_2 cluster bound by two cysteine persulfide residues. This observation lends structural support to a very recent Raman study, which reported that Fe_4S_4-to-Fe_2S_2 cluster conversion upon oxygen exposure in FNR resulted in concomitant production of cysteine persulfide as cluster ligands. Similar persulfide ligands have been observed in vitro for several other Fe_4S_4 cluster-containing proteins. We have also monitored FeS cluster conversion directly in our protein crystals. Our structures indicate that the Fe_4S_4-to-Fe_2S _2 change requires large structural modifications, which are most likely responsible for the dimer-monomer transition in FNR.

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《Proceedings of the National Academy of Sciences of the United States of America》 |2013年第18期|7188-7192|共5页
作者
Nicolet Y.; Rohac R.; Martin L.Fontecilla-Camps J.C.;
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作者单位

Metalloproteins Unit, Institut de Biologie Structurale J.-P. Ebel, Commissariat A l'Ener. Atomique-Ctr. National de la Recherche Scientifique-Universite Grenoble-Alpes, 38027 Grenoble, France;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
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FeFe-hydrogenase; X-ray crystallography;

X-ray snapshots of possible intermediates in the time course of synthesis and degradation of protein-bound Fe4S4 clusters

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