The architecture of the human Rad54-DNA complex provides evidence for protein translocation along DNA.

Ristic D; Wyman C; Paulusma CKanaar R

首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >The architecture of the human Rad54-DNA complex provides evidence for protein translocation along DNA.

【24h】

The architecture of the human Rad54-DNA complex provides evidence for protein translocation along DNA.

机译：The architecture of the human Rad54-DNA complex provides evidence for protein translocation along DNA.

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

Proper maintenance and duplication of the genome require accurate recombination between homologous DNA molecules. In eukaryotic cells, the Rad51 protein mediates pairing between homologous DNA molecules. This reaction is assisted by the Rad54 protein. To gain insight into how Rad54 functions, we studied the interaction of the human Rad54 (hRad54) protein with double-stranded DNA. We have recently shown that binding of hRad54 to DNA induces a change in DNA topology. To determine whether this change was caused by a protein-constrained change in twist, a protein-constrained change in writhe, or the introduction of unconstrained plectonemic supercoils, we investigated the hRad54--DNA complex by scanning force microscopy. The architecture of the observed complexes suggests that movement of the hRad54 protein complex along the DNA helix generates unconstrained plectonemic supercoils. We discuss how hRad54-induced superhelical stress in the target DNA may function to facilitate homologous DNA pairing by the hRad51 protein directly. In addition, the induction of supercoiling by hRad54 could stimulate recombination indirectly by displacing histones and/or other proteins packaging the DNA into chromatin. This function of DNA translocating motors might be of general importance in chromatin metabolism.

著录项

来源
《Proceedings of the National Academy of Sciences of the United States of America》 |2001年第15期|8454-8460|共7页
作者
Ristic D; Wyman C; Paulusma CKanaar R;
展开▼
作者单位

Department of Cell Biology and Genetics, Center for Biomedical Genetics, Erasmus University Rotterdam, PO Box 1738, 3000 DR Rotterdam, The Netherlands.;

hylo.life.uiuc.edu;

展开▼
收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
Adenosinetriphosphatase; DNA; DNA-Binding Proteins; Nuclear Proteins; Superhelical; 腺苷三磷酸酶; DNA结合蛋白质类; 核蛋白质类;

The architecture of the human Rad54-DNA complex provides evidence for protein translocation along DNA.

摘要

著录项

相关主题

期刊订阅