Targeted gene knockout in chickens mediated by TALENs

Tae Sub Park; Hong Jo Lee; Ki Hyun KimJin-Soo KimJae Yong Han

首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Targeted gene knockout in chickens mediated by TALENs

【24h】

Targeted gene knockout in chickens mediated by TALENs

机译：Targeted gene knockout in chickens mediated by TALENs

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

Genetically modified animals are used for industrial applications as well as scientific research, and studies on these animals contribute to a better understanding of biological mechanisms. Gene targeting techniques have been developed to edit specific gene loci in the genome, but the conventional strategy of homologous recombination with a gene-targeted vector has low efficiency and many technical complications. Here, we generated specific gene knockout chickens through the use of transcription activator-like effector nuclease (TALEN)-mediated gene targeting. In this study, we accomplished targeted knockout of the ovalbumin (OV) gene in the chicken primordial germ cells, and OV gene mutant offspring were generated through test-cross analysis. TALENs successfully induced nucleotide deletion mutations of ORF shifts, resulting in loss of chicken OV gene function. Our results demonstrate that the TALEN technique used in the chicken primordial germ cell line is a powerful strategy to create specific genome-edited chickens safely for practical applications.

著录项

来源
《Proceedings of the National Academy of Sciences of the United States of America》 |2014年第35期|12716-12721|共6页
作者
Tae Sub Park; Hong Jo Lee; Ki Hyun KimJin-Soo KimJae Yong Han;
展开▼
作者单位

Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Korea;

Department of Chemistry, College of Natural Science, Seoul National University, Seoul 151-921, Korea;

展开▼
收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
chickens; mediated; TALENs;

Targeted gene knockout in chickens mediated by TALENs

摘要

著录项

相关主题

期刊订阅