Inkjet-printed point-of-care immunoassay on a nanoscale polymer brush enables subpicomolar detection of analytes in blood

Joh Daniel Y.; Hucknall Angus M.; Wei QingshanMason Kelly A.Lund Margaret L.Fontes Cassio M.Hill Ryan T.Blair RebeccaZimmers ZackaryAchar Rohan K.Tseng DerekGordan RalucaFreemark MichaelOzcan AydoganChilkoti Ashutosh

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Inkjet-printed point-of-care immunoassay on a nanoscale polymer brush enables subpicomolar detection of analytes in blood

机译：Inkjet-printed point-of-care immunoassay on a nanoscale polymer brush enables subpicomolar detection of analytes in blood

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The ELISA is the mainstay for sensitive and quantitative detection of protein analytes. Despite its utility, ELISA is time-consuming, resource-intensive, and infrastructure-dependent, limiting its availability in resource-limited regions. Here, we describe a self-contained immunoassay platform (the "D4 assay") that converts the sandwich immunoassay into a point-of-care test (POCT). The D4 assay is fabricated by inkjet printing assay reagents as microarrays on nanoscale polymer brushes on glass chips, so that all reagents are "on-chip," and these chips show durable storage stability without cold storage. The D4 assay can interrogate multiple analytes from a drop of blood, is compatible with a smartphone detector, and displays analytical figures of merit that are comparable to standard laboratory-based ELISA in whole blood. These attributes of the D4 POCT have the potential to democratize access to high-performance immunoassays in resource-limited settings without sacrificing their performance.

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《Proceedings of the National Academy of Sciences of the United States of America.》 |2017年第34期|E7054-E7062|共9页
作者
Joh Daniel Y.; Hucknall Angus M.; Wei QingshanMason Kelly A.Lund Margaret L.Fontes Cassio M.Hill Ryan T.Blair RebeccaZimmers ZackaryAchar Rohan K.Tseng DerekGordan RalucaFreemark MichaelOzcan AydoganChilkoti Ashutosh;
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作者单位

Duke Univ, Pratt Sch Engn, Dept Biomed Engn, Durham, NC 27708 USA;

Univ Calif Los Angeles, Elect Engn Dept, Henry Samueli Sch Engn & Appl Sci, Los Angeles, CA 90095 USA;

Duke Univ, Ctr Genom & Computat Biol, Durham, NC 27708 USADuke Univ, Sch Med, Dept Pediat, Div Pediat Endocrinol, Durham, NC 27705 USA;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
nanoscale; nonfouling; polymer brush; inkjet printing; point of care;

Inkjet-printed point-of-care immunoassay on a nanoscale polymer brush enables subpicomolar detection of analytes in blood

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