Circadian clock-dependent and -independent rhythmic proteomes implement distinct diurnal functions in mouse liver

Daniel Mauvoisin; Jingkui Wang; Céline JouffeEva MartinFlorian AtgerPatrice WaridelManfredo QuadroniFrédéric GachonFelix Naef

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Circadian clock-dependent and -independent rhythmic proteomes implement distinct diurnal functions in mouse liver

机译：Circadian clock-dependent and -independent rhythmic proteomes implement distinct diurnal functions in mouse liver

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Diurnal oscillations of gene expression controlled by the circadian clock underlie rhythmic physiology across most living organisms. Although such rhythms have been extensively studied at the level of transcription and mRNA accumulation, little is known about the accumulation patterns of proteins. Here, we quantified temporal profiles in the murine hepatic proteome under physiological light–dark conditions using stable isotope labeling by amino acids quantitative MS. Our analysis identified over 5,000 proteins, of which several hundred showed robust diurnal oscillations with peak phases enriched in the morning and during the night and related to core hepatic physiological functions. Combinedmathematicalmodeling of temporal protein and mRNA profiles indicated that proteins accumulate with reduced amplitudes and significant delays, consistent with protein half-life data. Moreover, a group comprising about one-half of the rhythmic proteins showed no corresponding rhythmic mRNAs, indicating significant translational or posttranslational diurnal control. Such rhythms were highly enriched in secreted proteins accumulating tightly during the night. Also, these rhythms persisted in clock-deficient animals subjected to rhythmic feeding, suggesting that food-related entrainment signals influence rhythms in circulating plasma factors.

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《Proceedings of the National Academy of Sciences of the United States of America》 |2014年第1期|167-172|共6页
作者
Daniel Mauvoisin; Jingkui Wang; Céline JouffeEva MartinFlorian AtgerPatrice WaridelManfredo QuadroniFrédéric GachonFelix Naef;
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作者单位

Department of Pharmacology and Toxicology, University of Lausanne, CH-1005 Lausanne, Switzerland;

The Institute of Bioengineering, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne and Swiss Institute of Bioinformatics, Lausanne CH-1015, Switzerland;

Protein Analysis Facility, University of Lausanne, CH-1015 Lausanne, Switzerland;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
circadian rhythm; proteomics; liver metabolism; posttranslational regulation; protein secretion;

Circadian clock-dependent and -independent rhythmic proteomes implement distinct diurnal functions in mouse liver

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