Type IV pilins regulate their own expression via direct intramembrane interactions with the sensor kinase PilS

Kilmury Sara L. N.; Burrows Lori L.

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Type IV pilins regulate their own expression via direct intramembrane interactions with the sensor kinase PilS

机译：Type IV pilins regulate their own expression via direct intramembrane interactions with the sensor kinase PilS

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Type IV pili are important virulence factors for many pathogens, including Pseudomonas aeruginosa. Transcription of the major pilin gene-pilA-is controlled by the PilS-PilR two-component system in response to unknown signals. The absence of a periplasmic sensing domain suggested that PilS may sense an intramembrane signal, possibly PilA. We suggest that direct interactions between PilA and PilS in the inner membrane reduce pilA transcription when PilA levels are high. Overexpression in trans of PilA proteins with diverse and/or truncated C termini decreased native pilA transcription, suggesting that the highly conserved N terminus of PilA was the regulatory signal. Point mutations in PilA or PilS that disrupted their interaction prevented autoregulation of pilA transcription. A subset of PilA point mutants retained the ability to interact with PilS but could no longer decrease pilA transcription, suggesting that interaction between the pilin and sensor kinase is necessary but not sufficient for pilA autoregulation. Furthermore, PilS's phosphatase motif was required for the autoregulation of pilA transcription, suggesting that under conditions where PilA is abundant, the PilA-PilS interaction promotes PilR dephosphorylation and thus down-regulation of further pilA transcription. These data reveal a clever bacterial inventory control strategy in which the major subunit of an important P. aeruginosa virulence factor controls its own expression.

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《Proceedings of the National Academy of Sciences of the United States of America》 |2016年第21期|6017-6022|共6页
作者
Kilmury Sara L. N.; Burrows Lori L.;
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作者单位

McMaster Univ, Dept Biochem & Biomed Sci, Hamilton, ON L8S 4K1, Canada;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
type IV pili; two-component system; transcriptional regulation; Pseudomonas aeruginosa; Geobacter sulfurreducens;

Type IV pilins regulate their own expression via direct intramembrane interactions with the sensor kinase PilS

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